Bioactivity
(Glucose-6-phosphate dehydrogenase (G6PD) converts D-glucose 6-phosphate (G6P) into 6-phosphoglucono-delta-lactone and generate co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PD is the rate-limiting enzyme of the pentose phosphate pathway that supplies reducing energy to cells by maintaining the level of NADPH, which in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage from compounds like hydrogen peroxide. More importantly, NADPH is used for biosynthesis of fatty acids or isoprenoids. G6PD is generally found as a dimer of two identical monomers. Depending on conditions, such as pH, these dimers can themselves dimerize to form tetramers. Each monomer in the complex has a substrate binding site that binds to G6P, and a catalytic coenzyme binding site that binds to NADP /NADPH using the Rossman fold. Its activity is stimulated by the substrate G6P and NADP. The activity of recombinant human G6PD was measured by its ability to dehydrogenate glucose-6-phosphate. The reaction was performed in 25 mM Tris, 50 mM NaCl, 10 mM MgCl2, pH 8.0 (Assay Buffer), initiated by addition 50 uL of various concentrations of G6PD (diluted by Assay Buffer) to 50 ul substrate mixture consists of 1 mM NADP and 1 mM G6P. The final well serves as a negative control with no G6PD, replaced with 50 uL assay buffer. Then read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant human G6PD is >280 pmol/min/ug.)
Glucose-6-phosphate Dehydrogenase (G6PD) Active Protein | G6PD active protein
Active Glucose-6-phosphate Dehydrogenase (G6PD)
Gene Names
G6PD; G6PD1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Glucose-6-phosphate Dehydrogenase (G6PD); N/A; Active Glucose-6-phosphate Dehydrogenase (G6PD); Glucose-6-phosphate Dehydrogenase; Glucose-6-phosphate 1-dehydrogenase4; G6PD active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Met1~Leu515
Applicable Applications for G6PD active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.5
Research Area
Metabolic pathway
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Glucose-6-phosphate dehydrogenase (G6PD) converts D-glucose 6-phosphate (G6P) into 6-phosphoglucono-delta-lactone and generate co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PD is the rate-limiting enzyme of the pentose phosphate pathway that supplies reducing energy to cells by maintaining the level of NADPH, which in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage from compounds like hydrogen peroxide. More importantly, NADPH is used for biosynthesis of fatty acids or isoprenoids. G6PD is generally found as a dimer of two identical monomers. Depending on conditions, such as pH, these dimers can themselves dimerize to form tetramers. Each monomer in the complex has a substrate binding site that binds to G6P, and a catalytic coenzyme binding site that binds to NADP /NADPH using the Rossman fold. Its activity is stimulated by the substrate G6P and NADP. The activity of recombinant human G6PD was measured by its ability to dehydrogenate glucose-6-phosphate. The reaction was performed in 25 mM Tris, 50 mM NaCl, 10 mM MgCl2, pH 8.0 (Assay Buffer), initiated by addition 50 uL of various concentrations of G6PD (diluted by Assay Buffer) to 50 ul substrate mixture consists of 1 mM NADP and 1 mM G6P. The final well serves as a negative control with no G6PD, replaced with 50 uL assay buffer. Then read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant human G6PD is >280 pmol/min/ug.)
Bioactivity
(Glucose-6-phosphate dehydrogenase (G6PD) converts D-glucose 6-phosphate (G6P) into 6-phosphoglucono-delta-lactone and generate co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PD is the rate-limiting enzyme of the pentose phosphate pathway that supplies reducing energy to cells by maintaining the level of NADPH, which in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage from compounds like hydrogen peroxide. More importantly, NADPH is used for biosynthesis of fatty acids or isoprenoids. G6PD is generally found as a dimer of two identical monomers. Depending on conditions, such as pH, these dimers can themselves dimerize to form tetramers. Each monomer in the complex has a substrate binding site that binds to G6P, and a catalytic coenzyme binding site that binds to NADP /NADPH using the Rossman fold. Its activity is stimulated by the substrate G6P and NADP. The activity of recombinant human G6PD was measured by its ability to dehydrogenate glucose-6-phosphate. The reaction was performed in 25 mM Tris, 50 mM NaCl, 10 mM MgCl2, pH 8.0 (Assay Buffer), initiated by addition 50 uL of various concentrations of G6PD (diluted by Assay Buffer) to 50 ul substrate mixture consists of 1 mM NADP and 1 mM G6P. The final well serves as a negative control with no G6PD, replaced with 50 uL assay buffer. Then read at a wavelength of 340 nm in kinetic mode for 5 minutes. The specific activity of recombinant human G6PD is >280 pmol/min/ug.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for G6PD active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
59,257 Da
NCBI Official Full Name
G6PD
NCBI Official Synonym Full Names
glucose-6-phosphate dehydrogenase
NCBI Official Symbol
G6PD
NCBI Official Synonym Symbols
G6PD1
NCBI Protein Information
glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase, G6PD
UniProt Protein Name
Glucose-6-phosphate 1-dehydrogenase
UniProt Gene Name
G6PD
UniProt Synonym Gene Names
G6PD
UniProt Entry Name
G6PD_HUMAN
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Product Notes
The G6PD g6pd (Catalog #AAA161771) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glucose-6-phosphate Dehydrogenase (G6PD) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the G6PD g6pd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Leu51 5. It is sometimes possible for the material contained within the vial of "Glucose-6-phosphate Dehydrogenase (G6PD), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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