Glutathione S Transferase Pi (GSTp) Active Protein | GSTp active protein

Active Glutathione S Transferase Pi (GSTp)

Cat. Num. AAA148150

• Applications: Invitro Assay, Activity Assay, Cell Culture
• Purity: >95%
• Synonyms: Glutathione S Transferase Pi (GSTp); N/A; Active Glutathione S Transferase Pi (GSTp); GSTp active protein

• Host: E. coli
• Purity/Purification: >95%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl and 5% trehalose.

• Applicable Applications for GSTp active protein: Invitro Assay, Activity Assay, Cell Culture
• Source: Prokaryotic expression.
• Residues: Met1~Gln210
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Predicted isoelectric point: 6.2
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8°C for one month. ; Aliquot and store at -80°C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Western Blot; Sample: Recombinant GSTp, HumanAntibody: Rabbit Anti-Human GSTp Ab )

SDS_PAGE

(SDS-PAGE ImageSample: Active Recombinant GSTp, Human)

Gene Sequencing

(Gene Sequencing (extract))

Bioactivity

Sequence

Related Product Information for GSTp active protein

GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTp and CDK5 was shown in Figure 1, and this effect was in a dose dependent manner.

Product Categories/Family for GSTp active protein

Enzyme & Kinase; Metabolic pathway; Tumor immunity; Hepatology

NCBI and Uniprot Product Information

• UniProt Accession #: P09211; O00460; Q15690; Q5TZY3
• Molecular Weight: Predicted Molecular Mass: 24.6kDa; Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
• UniProt Protein Name: Glutathione S-transferase P
• UniProt Gene Name: GSTP1
• UniProt Synonym Gene Names: FAEES3; GST3