IGF-I Active Protein | IGF1 active protein
Fish (denis) IGF-I
Gene Names
IGF1; IGFI; IGF-I; IGF1A
Purity
Greater than 98.0% as determined by:(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Synonyms
IGF-I; N/A; Fish (denis) IGF-I; Fish gilthead seabream (Sparus aurata) IGF-I; IGF1 active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Form/Format
White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ser-Pro-Glu-Thr-Leu-Cys-Gly-Ala-Glu.
Sequence Length
137
Solubility
It is recommended to reconstitute the lyophilized gsIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.60 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
Dimers and Aggregates
The purified gsIGF-I consists of > 97% monomers as determined by gel-filtration chromatography.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of Gilthead Seabream IGF-I
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Biological Activity
Binding assays of the 125I-gsIGF-I to gilthead seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude gilthead seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF-I was about fourfold lower than that of gsIGF-I. Recombinant gsIGF-I exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF-1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF-I receptor in these cells. In contrast, the activities of gsIGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant gsIGF-I is biologically active.
Preparation and Storage
Lyophilized gsIGF-I although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 gsIGF-I mg/ml and up to 2 2 mg/ml and filter sterilization gsIGF-I can be stored at +4 degree C
Related Product Information for IGF1 active protein
Gilthead seabream (Sparus aurata) insulin-like growth factor-I (gsIGF-I) cDNA coding the mature protein was expressed in Escherichia coli. The expressed protein contained within the inclusion-body pellet was solubilized and purified to over 98% purity, as a monomeric methionyl-gsIGF-I. Amino acid composition and N-terminal sequence confirmed the identity to be the predicted protein. For reference see Fine et al., J Endocrinol. 1997 53:139-150.
Product Categories/Family for IGF1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
insulin-like growth factor I
NCBI Official Synonym Full Names
insulin-like growth factor 1 (somatomedin C)
NCBI Official Symbol
IGF1
NCBI Official Synonym Symbols
IGFI; IGF-I; IGF1A
NCBI Protein Information
insulin-like growth factor I; IGF-IA; IGF-IB; MGF; insulin-like growth factor IA; insulin-like growth factor IB; mechano growth factor; somatomedin-C
UniProt Protein Name
Insulin-like growth factor I
UniProt Gene Name
IGF1
UniProt Synonym Gene Names
IBP1; IGF-I; MGF
UniProt Entry Name
IGF1_HUMAN
Similar Products
Product Notes
The IGF1 igf1 (Catalog #AAA60570) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first ten N-terminal amino acids was determined and was found to be Met-Ser-Pr o-Glu-Thr- Leu-Cys-Gl y-Ala-Glu. It is sometimes possible for the material contained within the vial of "IGF-I, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.