Application Data
IL3 active protein
Recombinant Human IL3
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Purity
>95% as determined by SDS-PAGE
Synonyms
IL3; N/A; Recombinant Human IL3; IL-3; Mast cell growth factor; MCGF; Multi-CSF; Hematopoietic growth factor; HCGF; P-cell stimulation factor; Recombinant Human Interleukin-3 (IL3); IL3 active protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from a 0.2 um filtered solution in Phosphate Buffer and NaCl (pH 6.3)
Sequence Length
152
Bioactivity
The ED50 as determined by the dose-dependent proliferation of Human TF-1 cells was found less than 0.5 ng/mL
Endotoxin Level
<1.0 EU/ug of recombinant protein as determined by the LAL method
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for IL3 active protein
IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial-derived recombinant IL3 shows that glycosylation is not required for activity. IL3 sequences are evolutionarily less well conserved with human and murine IL3 sharing approximately 29% homology (at the protein level) and murine and rat IL3 sharing approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant human IL3 is a non-glycosylated globular protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
15.0 kDa
NCBI Official Full Name
IL-3
NCBI Official Synonym Full Names
interleukin 3
NCBI Official Symbol
IL3
NCBI Official Synonym Symbols
IL-3; MCGF; MULTI-CSF
NCBI Protein Information
interleukin-3
UniProt Protein Name
Interleukin-3
UniProt Gene Name
IL3
UniProt Synonym Gene Names
IL-3; MCGF
UniProt Entry Name
IL3_HUMAN
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Product Notes
The IL3 il3 (Catalog #AAA214236) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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