Bioactivity
(Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of sixIGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structuresand bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Withintissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and blocktheir access to the IGF-1 receptor (IGF1R), which is activated by both IGFs.IGFBP3 also interacts with cell-surface proteins, affecting cell signaling fromoutside the cell or after internalization, and also enters the cell nucleus where itbinds to nuclear hormone receptors and other ligands. Besides, Fibronectin (FN)has been identified as an interactor of IGFBP3, thus a binding ELISA assay wasconducted to detect the interaction of recombinant human IGFBP3 andrecombinant human FN.Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicatesamples of 100ul were then transferred to FN-coated microtiter wells andincubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1hwith anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation withHRP labelled secondary antibody, wells were aspirated and washed 5 times.With the addition of substrate solution, wells were incubated 15-25 minutes at37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately.The binding activity of IGFBP3 and FN was shown in Figure 1, the EC50 was 78ng/ml.)
Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Active Protein | IGFBP3 active protein
Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3)
Gene Names
IGFBP3; IBP3; BP-53
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Insulin Like Growth Factor Binding Protein 3 (IGFBP3); N/A; Active Insulin Like Growth Factor Binding Protein 3 (IGFBP3); Insulin Like Growth Factor Binding Protein 3; BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex4; IGFBP3 active protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Gly135~Thr277
Applicable Applications for IGFBP3 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
Two N-terminal Tags, His-tag and SUMO-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
9.3
Research Area
Metabolic pathway, Tumor immunity
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of sixIGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structuresand bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Withintissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and blocktheir access to the IGF-1 receptor (IGF1R), which is activated by both IGFs.IGFBP3 also interacts with cell-surface proteins, affecting cell signaling fromoutside the cell or after internalization, and also enters the cell nucleus where itbinds to nuclear hormone receptors and other ligands. Besides, Fibronectin (FN)has been identified as an interactor of IGFBP3, thus a binding ELISA assay wasconducted to detect the interaction of recombinant human IGFBP3 andrecombinant human FN.Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicatesamples of 100ul were then transferred to FN-coated microtiter wells andincubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1hwith anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation withHRP labelled secondary antibody, wells were aspirated and washed 5 times.With the addition of substrate solution, wells were incubated 15-25 minutes at37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately.The binding activity of IGFBP3 and FN was shown in Figure 1, the EC50 was 78ng/ml.)
Bioactivity
(Insulin-like growth factor-binding protein 3, also known as IGFBP3 is one of sixIGF binding proteins (IGFBP1 to IGFBP6) that have highly conserved structuresand bind the insulin-like growth factors IGF-1 and IGF-2 with high affinity. Withintissues, IGFBP3 can bind IGF1 and IGF2 released by many cell types, and blocktheir access to the IGF-1 receptor (IGF1R), which is activated by both IGFs.IGFBP3 also interacts with cell-surface proteins, affecting cell signaling fromoutside the cell or after internalization, and also enters the cell nucleus where itbinds to nuclear hormone receptors and other ligands. Besides, Fibronectin (FN)has been identified as an interactor of IGFBP3, thus a binding ELISA assay wasconducted to detect the interaction of recombinant human IGFBP3 andrecombinant human FN.Briefly, IGFBP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicatesamples of 100ul were then transferred to FN-coated microtiter wells andincubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1hwith anti-IGFBP3 pAb, then aspirated and washed 3 times. After incubation withHRP labelled secondary antibody, wells were aspirated and washed 5 times.With the addition of substrate solution, wells were incubated 15-25 minutes at37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately.The binding activity of IGFBP3 and FN was shown in Figure 1, the EC50 was 78ng/ml.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for IGFBP3 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,674 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 3 isoform b
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 3
NCBI Official Symbol
IGFBP3
NCBI Official Synonym Symbols
IBP3; BP-53
NCBI Protein Information
insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex
UniProt Protein Name
Insulin-like growth factor-binding protein 3
UniProt Gene Name
IGFBP3
UniProt Synonym Gene Names
IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
UniProt Entry Name
IBP3_HUMAN
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Product Notes
The IGFBP3 igfbp3 (Catalog #AAA161673) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Insulin Like Growth Factor Binding Protein 3 (IGFBP3) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the IGFBP3 igfbp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gly135~Thr 277. It is sometimes possible for the material contained within the vial of "Insulin Like Growth Factor Binding Protein 3 (IGFBP3), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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