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Application Data
Interferon Gamma (IFNg) Active Protein | IFNg active protein
Active Interferon Gamma (IFNg)
Gene Names
IFNG; IFG; IFI
Applications
Activity Assay, Cell Culture
Purity
> 95%
Synonyms
Interferon Gamma (IFNg); N/A; Active Interferon Gamma (IFNg); IFN-G; IFG; IFI; INFr; IFN, Immune Interferon; IFNg active protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Freeze-dried powder
Sequence Length
166
Applicable Applications for IFNg active protein
Activity Assay, Cell Culture
Fragment
Gln24~Gln166
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Organism Species
Human
Isoelectric Point
9.7
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
Application Data
Application Data
Application Data
SDS-PAGE
SDS-PAGE
Activity
(IFN-gamma is a dimerized soluble cytokine that is the only member of the type II class of interferons.The importance of IFNgamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. As reported, IFNgamma is an important activator of human monocytic THP1 cells. Therefore,THP-1 cells were incubated in RPMI 1640 with various concentration of IFN-gamma, then cells were observed by inverted microscope everyday.After stimulated with IFN-gamma(5ng/ml) for 5 days, morphological changes occured in THP1 cells which displayed the shape of fusiform or polygon and were more likely to adhere.Effect of IFN-gamma on THP1 cells is shown in Figure 1.)
Activity
(IFN-gamma is a dimerized soluble cytokine that is the only member of the type II class of interferons.The importance of IFNgamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. As reported, IFNgamma is an important activator of human monocytic THP1 cells. Therefore,THP-1 cells were incubated in RPMI 1640 with various concentration of IFN-gamma, then cells were observed by inverted microscope everyday.After stimulated with IFN-gamma(5ng/ml) for 5 days, morphological changes occured in THP1 cells which displayed the shape of fusiform or polygon and were more likely to adhere.Effect of IFN-gamma on THP1 cells is shown in Figure 1.)
Product Categories/Family for IFNg active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18.4kDa; 22&25kDa
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon gamma
NCBI Official Symbol
IFNG
NCBI Official Synonym Symbols
IFG; IFI
NCBI Protein Information
interferon gamma
UniProt Protein Name
Interferon gamma
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
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Product Notes
The IFNg ifng (Catalog #AAA146601) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Interferon Gamma (IFNg) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the IFNg ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interferon Gamma (IFNg), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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