SDS-PAGE
Interleukin-3 Active Protein | IL3 active protein
Interleukin-3, 20-152aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
IL3; IL-3; MCGF; MULTI-CSF
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Interleukin-3; N/A; Interleukin-3, 20-152aa, Human, His tag, E Coli (Bioactivity Validated); IL-3; Multipotential colony-stimulating factor (multi-CSF); Mast cell growth factor (MCGF); interleukin 3 (colony-stimulating factor; multiple); MuLTI-CSF; MCGF; multilineage-colony-stimulating factor; hematopoietic growth factor; P-cell stimulating factor; mast-cell growth factor; IL3 active protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid in 20mM Tris pH 8.0, 0.2mM PMSF, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
Sequence Length
152
Applicable Applications for IL3 active protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects less or equal to 1 ng/ml.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for IL3 active protein
Interleukin-3 (IL-3) stimulates the proliferation of hematopoietic pluripotent progenitor cells. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 exerts its biological activities through binding to specific cell surface receptors. IL-3 receptor is a complex consisting of alpha and beta subunits, which in turn induces activation of JAK2/ STAT5, induction of c-myc, and activation of the Ras pathway. Human IL3 is a protein of 15-17 kDa (133 amino acids). It is synthesized as a precursor containing a hydrophobic secretory signal sequence of 19 amino acids. IL3 contains two putative glycosylation sites at positions 15 and 70 and contains a single disulfide bond (Cys16/84). Recombinant human IL-3 was expressed in E Coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
Product Categories/Family for IL3 active protein
References
Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.; Michaud DS., et al. (2006), Cancer Res. 66 (8):4525-30;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17.3kDa (154aa), confirmed by MALDI-TOF.
NCBI Official Full Name
IL-3
NCBI Official Synonym Full Names
interleukin 3
NCBI Official Symbol
IL3
NCBI Official Synonym Symbols
IL-3; MCGF; MULTI-CSF
NCBI Protein Information
interleukin-3
UniProt Protein Name
Interleukin-3
UniProt Gene Name
IL3
UniProt Synonym Gene Names
IL-3; MCGF
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Product Notes
The IL3 il3 (Catalog #AAA48476) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Interleukin-3 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the IL3 il3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF. It is sometimes possible for the material contained within the vial of "Interleukin-3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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