Application Data
(Biological Acitivity: Human IL-6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )
Interleukin 6 Active Protein | IL6 active protein
Interleukin 6, 30-212aa, Human, His tag, E Coli (Bioactivity Validated)
Gene Names
IL6; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Interleukin 6; N/A; Interleukin 6, 30-212aa, Human, His tag, E Coli (Bioactivity Validated); HGF; HSF; BSF2; IL-6; IFNB2; IL6; interleukin 6 (interferon; beta 2),; IL6 active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM
Sequence Length
212
Applicable Applications for IL6 active protein
Bioactivity, SDS-PAGE
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.8 ng/ml.
Preparation and Storage
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
Application Data
(Biological Acitivity: Human IL-6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )
Application Data
(Biological Acitivity: Human IL-6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range )
SDS-PAGE
SDS-PAGE
Related Product Information for IL6 active protein
Interleukin-6, as known as IL-6, is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is also a 'myokine,' a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6. It is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. Recombinant interleukin-6 was expressed in E Coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for IL6 active protein
References
Ishihara, et al. (2002) Cytokine Growth Factor Rev. 13 (4-5):357-68. ; Vgontzas AN, et al. (2005) Neuroimmunomodulation.12, 131-40.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23.1 kDa (204 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
interleukin-6 isoform 1
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
NCBI Protein Information
interleukin-6
UniProt Protein Name
Interleukin-6
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
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Product Notes
The IL6 il6 (Catalog #AAA48546) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's Interleukin 6 can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM. It is sometimes possible for the material contained within the vial of "Interleukin 6, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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