Bioactivity
(Kallistatin (KAL), a member of serpin family, can Inhibit human amidolytic and kininogenase activities of tissue kallikrein. KAL is widely distributed in a variety of organs and body fluids and has biological effects such as anti-inflammatory, antioxidant stress, anti-angiogenesis and anti-tumor. Besides, Kallikrein 2 (KLK2) has been identified as an interactor of KAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human KAL and recombinant human KLK2. Briefly, KLK2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to KAL-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-KLK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. When recombinant human KAL is immobilized at 2 ug/mL (100 uL/well), the concentration of KLK2 that produces 50% optimal binding response is found to be approximately 0.49 ug/mL.)
Kallistatin (KAL) Active Protein | KAL active protein
Active Kallistatin (KAL)
Gene Names
SERPINA4; KAL; KST; PI4; KLST; PI-4; kallistatin
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Synonyms
Kallistatin (KAL); N/A; Active Kallistatin (KAL); Kallistatin; SERPINA4; KLST; KST; PI4; Serpin Peptidase Inhibitor Clade A Member 4; Tissue Kallikrein Inhibitor; Kallikrein Binding Protein; Peptidase inhibitor 44; KAL active protein
Host
E Coli
Purity/Purification
Greater than 80% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Gln21~Pro427
Applicable Applications for KAL active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
8.1
Research Area
Infection immunity
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Kallistatin (KAL), a member of serpin family, can Inhibit human amidolytic and kininogenase activities of tissue kallikrein. KAL is widely distributed in a variety of organs and body fluids and has biological effects such as anti-inflammatory, antioxidant stress, anti-angiogenesis and anti-tumor. Besides, Kallikrein 2 (KLK2) has been identified as an interactor of KAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human KAL and recombinant human KLK2. Briefly, KLK2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to KAL-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-KLK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. When recombinant human KAL is immobilized at 2 ug/mL (100 uL/well), the concentration of KLK2 that produces 50% optimal binding response is found to be approximately 0.49 ug/mL.)
Bioactivity
(Kallistatin (KAL), a member of serpin family, can Inhibit human amidolytic and kininogenase activities of tissue kallikrein. KAL is widely distributed in a variety of organs and body fluids and has biological effects such as anti-inflammatory, antioxidant stress, anti-angiogenesis and anti-tumor. Besides, Kallikrein 2 (KLK2) has been identified as an interactor of KAL, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human KAL and recombinant human KLK2. Briefly, KLK2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to KAL-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-KLK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. When recombinant human KAL is immobilized at 2 ug/mL (100 uL/well), the concentration of KLK2 that produces 50% optimal binding response is found to be approximately 0.49 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for KAL active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,542 Da
NCBI Official Full Name
kallistatin isoform 2
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
NCBI Official Symbol
SERPINA4
NCBI Official Synonym Symbols
KAL; KST; PI4; KLST; PI-4; kallistatin
NCBI Protein Information
kallistatin; serpin A4; kallikrein inhibitor; peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4
UniProt Protein Name
Kallistatin
UniProt Gene Name
SERPINA4
UniProt Synonym Gene Names
KST; PI4; PI-4
UniProt Entry Name
KAIN_HUMAN
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Product Notes
The KAL serpina4 (Catalog #AAA161912) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Kallistatin (KAL) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the KAL serpina4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gln21~Pro4 27. It is sometimes possible for the material contained within the vial of "Kallistatin (KAL), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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