MMP-1 active protein
Human MMP-1
Gene Names
MMP1; CLG; CLGN
Purity
> 98% by SDS-PAGE & HPLC analysis
Synonyms
MMP-1; N/A; Human MMP-1; Matrix metalloproteinase-1; fibroblast collagenase; interstitial collagenase; MMP-1 active protein
Host
E Coli
Purity/Purification
> 98% by SDS-PAGE & HPLC analysis
Form/Format
Lyophilized
Sequence
MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNV TPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG P GIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGAL MYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DS KLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPN GL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFP RTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAH D FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANS WFNCRK N
Sequence Length
469
Endotoxin Level
<0.1 ng/ug of protein (<1 EU/ug)
Length (aa)
371
Biological Activity
MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 ug/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes
Reconstitution
Reconstitute in water to a concentration of 0.5-1.0mg/m. Do not vortex. Note: Slow to dissolve. Gently shake the solution for about 10 minutes for better solubility. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and to store in working aliquots at -20°C to -80°C.
Preparation and Storage
The lyophilized protein is stable at room temperature for 1 month at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C for 3 months at -20°C to -80°C.
Related Product Information for MMP-1 active protein
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-1 is a secreted collagenase with specificity toward Type I, II, III, VII, and X collagens. Recombinant human MMP-1 is a 42.7 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain which is involved in substrate specificity and in binding TIMP-1.
Product Categories/Family for MMP-1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42.7 kDa
NCBI Official Full Name
interstitial collagenase isoform 1 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 1 (interstitial collagenase)
NCBI Official Symbol
MMP1
NCBI Official Synonym Symbols
CLG; CLGN
NCBI Protein Information
interstitial collagenase; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1
UniProt Protein Name
Interstitial collagenase
UniProt Gene Name
MMP1
UniProt Synonym Gene Names
CLG; MMP-1
UniProt Entry Name
MMP1_HUMAN
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Product Notes
The MMP-1 mmp1 (Catalog #AAA79296) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MFVLTEGNPR WEQTHLTYRI ENYTPDLPRA DVDHAIEKAF QLWSNV TPLT FTKVSEGQAD IMISFVRGDH RDNSPFDGPG GNLAHAFQPG P GIGGDAHFD EDERWTNNFR EYNLHRVAAH ELGHSLGLSH STDIGAL MYP SYTFSGDVQL AQDDIDGIQA IYGRSQNPVQ PIGPQTPKAC DS KLTFDAIT TIRGEVMFFK DRFYMRTNPF YPEVELNFIS VFWPQLPN GL EAAYEFADRD EVRFFKGNKY WAVQGQNVLH GYPKDIYSSF GFP RTVKHID AALSEENTGK TYFFVANKYW RYDEYKRSMD PGYPKMIAH D FPGIGHKVDA VFMKDGFFYF FHGTRQYKFD PKTKRILTLQ KANS WFNCRK N. It is sometimes possible for the material contained within the vial of "MMP-1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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