SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Osteopontin/OPN protein Active Protein | OPN active protein
Recombinant human Osteopontin/OPN protein
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
Osteopontin/OPN protein; N/A; Recombinant human Osteopontin/OPN protein; OPN; SPP-1; BNSP; BSPI; ETA-1; Bone sialoprotein 1; BSP I.Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; Secreted phosphoprotein 1; SPP 1; SPP1; urinary stone protein; uropontin;; OPN active protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 2mM EDTA
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
17-314aa
Sequence
< MGSSHHHHHH SSGLVPRGSH > RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N
Applicable Applications for OPN active protein
Bioactivity, SDS-PAGE
Antigen Species
Human
Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to OPN coated plates 10ug/ml. This effect is more to 40%.
Tag Information
His-tag
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for OPN active protein
Description: Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
References
Jung SH, et al. On-chip dual enzyme activity assay to investigate regulation of the transamidase and kinase activities of transglutaminase 2. Anal Chim Acta. 2018 Oct 16;1027:92-100.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
36.2 kDa (321aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Similar Products
Product Notes
The OPN (Catalog #AAA48974) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 17-314aa with tag His-tag. AAA Biotech's Osteopontin/OPN protein can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the OPN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: < MGSSHHHHHH SSGLVPRGSH > RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N. It is sometimes possible for the material contained within the vial of "Osteopontin/OPN protein, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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