Bioactivity
(Human Prolactin in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range is less than or equal to 0.5ng/ml.)
Prolactin Active Protein | PRL active protein
Prolactin, 29-227aa, Human, E Coli (Bioactivity Validated)
Gene Names
PRL; GHA1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
Prolactin; N/A; Prolactin, 29-227aa, Human, E Coli (Bioactivity Validated); PRL; Lactogenic hormone; Luteotropic hormone; Mammotropin; OTTHuMP00000017727; Prolactin precursor.; PRL active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffer saline (pH 7.4)
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MLPICPGGAA RCQVTLRDLF DRAVVLSHYI HNLSSEMFSE FDKRYTHGRG FITKAINSCH TSSLATPEDK EQAQQMNQKD FLSLIVSILR SWNEPLYHLV TEVRGMQEAP EAILSKAVEI EEQTKRLLEG MELIVSQVHP ETKENEIYPV WSGLPSLQMA DEESRLSAYY NLLHCLRRDS HKIDNYLKLL KCRIIHNNNC
Sequence Length
227
Applicable Applications for PRL active protein
Bioactivity, SDS-PAGE
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 for this effect is less or equal to 0.5 ng/ml.
Preparation and Storage
Can be stored at +2 to +8 degrees C for 1 week.
For long term storage, aliquot and store at -20 degrees C to -80 degrees C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degrees C to -80 degrees C.
Avoid repeated freezing and thawing cycles.
Bioactivity
(Human Prolactin in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range is less than or equal to 0.5ng/ml.)
Bioactivity
(Human Prolactin in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range is less than or equal to 0.5ng/ml.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for PRL active protein
Prolactin is a hormone synthesised and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland). Prolactin has many effects, the most significant of which is to stimulate the mammary glands to produce milk (lactation).Increased serum prolactin during pregnancy causes enlargement of the mammary glands of the breasts and increases the production of milk. Recently, prolactin has demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. Prolactin was expressed in E Coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for PRL active protein
References
Ben-jonathan N., et al. (1996) Endocr Rev. 17 (6):639-69.; Cooke NE., et al. (1981) J. Biol. Chem. 256, 4007-4016.; Goffin V., et al. (1999) Mol Cell Endocrinol. 151, 79-87.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23 kDa (200 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
PRL
NCBI Official Synonym Symbols
GHA1
NCBI Protein Information
prolactin
UniProt Protein Name
Prolactin
UniProt Gene Name
PRL
UniProt Synonym Gene Names
PRL
Similar Products
Product Notes
The PRL prl (Catalog #AAA48461) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Prolactin can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the PRL prl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLPICPGGAA RCQVTLRDLF DRAVVLSHYI HNLSSEMFSE FDKRYTHGRG FITKAINSCH TSSLATPEDK EQAQQMNQKD FLSLIVSILR SWNEPLYHLV TEVRGMQEAP EAILSKAVEI EEQTKRLLEG MELIVSQVHP ETKENEIYPV WSGLPSLQMA DEESRLSAYY NLLHCLRRDS HKIDNYLKLL KCRIIHNNNC. It is sometimes possible for the material contained within the vial of "Prolactin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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