Bioactivity
(Protein Z-dependent Protease Inhibitor (ZPI), also known as SerpinA10 (SERine Proteinase INhibitor-clade A10) is a monomeric, secreted member of the A (or extracellular) clade within the serpin superfamily of protease inhibitors. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpins are unusual in that they are one-time use, non-recyclable proteins whose native state is thermodynamically unstable. The activity of recombinant human SERPINA10 was measured by its ability to inhibit Coagulation Factor X cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Coagulation Factor X was diluted to 5 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human SERPINA10 (MW: 78.26 KD) was incubated with 25 ul diluted Coagulation Factor X at 37 degree C for 30 minutes. Loading 50 uL 20 uM substrate to start the reaction including a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human SERPINA10 significantly decreased Coagulation Factor X activity. The inhibition IC50 was )
Serpin A10 (SERPINA10) Active Protein | SERPINA10 active protein
Active Serpin A10 (SERPINA10)
Gene Names
SERPINA10; PZI; ZPI
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Synonyms
Serpin A10 (SERPINA10); N/A; Active Serpin A10 (SERPINA10); Serpin A10; PZI; ZPI; Serine(Cysteine)Proteinase Inhibitor,Clade A(Alpha-1 Antiproteinase,Antitrypsin)Member 10; Protein Z-Related Protease Inhibitor; Protein Z Dependent Protease Inhibitor4; SERPINA10 active protein
Host
E Coli
Purity/Purification
Greater than 80% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Pro24~Leu444
Applicable Applications for SERPINA10 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His and GST Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
8.1
Research Area
Enzyme & Kinase, Metabolic pathway, Hematology
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Protein Z-dependent Protease Inhibitor (ZPI), also known as SerpinA10 (SERine Proteinase INhibitor-clade A10) is a monomeric, secreted member of the A (or extracellular) clade within the serpin superfamily of protease inhibitors. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpins are unusual in that they are one-time use, non-recyclable proteins whose native state is thermodynamically unstable. The activity of recombinant human SERPINA10 was measured by its ability to inhibit Coagulation Factor X cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Coagulation Factor X was diluted to 5 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human SERPINA10 (MW: 78.26 KD) was incubated with 25 ul diluted Coagulation Factor X at 37 degree C for 30 minutes. Loading 50 uL 20 uM substrate to start the reaction including a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human SERPINA10 significantly decreased Coagulation Factor X activity. The inhibition IC50 was )
Bioactivity
(Protein Z-dependent Protease Inhibitor (ZPI), also known as SerpinA10 (SERine Proteinase INhibitor-clade A10) is a monomeric, secreted member of the A (or extracellular) clade within the serpin superfamily of protease inhibitors. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpins are unusual in that they are one-time use, non-recyclable proteins whose native state is thermodynamically unstable. The activity of recombinant human SERPINA10 was measured by its ability to inhibit Coagulation Factor X cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Coagulation Factor X was diluted to 5 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human SERPINA10 (MW: 78.26 KD) was incubated with 25 ul diluted Coagulation Factor X at 37 degree C for 30 minutes. Loading 50 uL 20 uM substrate to start the reaction including a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human SERPINA10 significantly decreased Coagulation Factor X activity. The inhibition IC50 was )
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for SERPINA10 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,707 Da
NCBI Official Full Name
protein Z-dependent protease inhibitor
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
NCBI Official Symbol
SERPINA10
NCBI Official Synonym Symbols
PZI; ZPI
NCBI Protein Information
protein Z-dependent protease inhibitor; serpin A10; PZ-dependent protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
UniProt Protein Name
Protein Z-dependent protease inhibitor
UniProt Gene Name
SERPINA10
UniProt Synonym Gene Names
ZPI; PZ-dependent protease inhibitor; PZI
UniProt Entry Name
ZPI_HUMAN
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Product Notes
The SERPINA10 serpina10 (Catalog #AAA161873) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Serpin A10 (SERPINA10) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the SERPINA10 serpina10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Pro24~Leu4 44. It is sometimes possible for the material contained within the vial of "Serpin A10 (SERPINA10), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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