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Sequence
Steroid 5 Alpha Reductase 1 (SRD5a1) Active Protein | SRD5a1 active protein
Active Steroid 5 Alpha Reductase 1 (SRD5a1)
Gene Names
Srd5a1; S5AR 1; Srd5a-1; 0610031P22Rik; 4930435F02Rik
Applications
Activity Assay
Purity
>80%
Synonyms
Steroid 5 Alpha Reductase 1 (SRD5a1); N/A; Active Steroid 5 Alpha Reductase 1 (SRD5a1); Steroid 5 Alpha Reductase 1; 3-Oxo-5 Alpha-Steroid Delta 4-Dehydrogenase Alpha 14; SRD5a1 active protein
Host
E. coli
Purity/Purification
>80%
Form/Format
PBS, pH 7.4, containing 0.01% SKL, 5%Trehalose
Concentration
Original Concentration: 300 ug/mL (varies by lot)
Applicable Applications for SRD5a1 active protein
Activity Assay
Source
Prokaryotic expression
Residues
Met1~Leu255
Tags
N-terminal His and GST Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
8.7
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Identification
Identification
SDS-PAGE
SDS-PAGE
Bioactivity
(Steroid 5 Alpha Reductase 1 (SRD5a1) a member of the steroid 5alpha-reductase family (SRD5A1, SRD5A2 and SRD5A3) converting testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. SRD5A1 was mainly expressed in the skin, scalp, liver, and brain tissues. Prostatic Adenoma and Prostatic Hyperplasia are associated with SRD5A1. Besides, 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) has been identified as an interactor of SRD5a1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse SRD5a1 and recombinant human HSD17b3. Briefly, biotin-linked SRD5a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HSD17b3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse SRD5a1 and recombinant human HSD17b3 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Bioactivity
(Steroid 5 Alpha Reductase 1 (SRD5a1) a member of the steroid 5alpha-reductase family (SRD5A1, SRD5A2 and SRD5A3) converting testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. SRD5A1 was mainly expressed in the skin, scalp, liver, and brain tissues. Prostatic Adenoma and Prostatic Hyperplasia are associated with SRD5A1. Besides, 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) has been identified as an interactor of SRD5a1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse SRD5a1 and recombinant human HSD17b3. Briefly, biotin-linked SRD5a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HSD17b3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse SRD5a1 and recombinant human HSD17b3 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for SRD5a1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Accurate Molecular Mass: 57/40/35/28kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
NCBI Official Synonym Full Names
steroid 5 alpha-reductase 1
NCBI Official Symbol
Srd5a1
NCBI Official Synonym Symbols
S5AR 1; Srd5a-1; 0610031P22Rik; 4930435F02Rik
NCBI Protein Information
3-oxo-5-alpha-steroid 4-dehydrogenase 1
UniProt Protein Name
3-oxo-5-alpha-steroid 4-dehydrogenase 1
UniProt Gene Name
Srd5a1
UniProt Synonym Gene Names
S5AR 1
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Product Notes
The SRD5a1 srd5a1 (Catalog #AAA161940) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Steroid 5 Alpha Reductase 1 (SRD5a1) can be used in a range of immunoassay formats including, but not limited to, Activity Assay. Researchers should empirically determine the suitability of the SRD5a1 srd5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Steroid 5 Alpha Reductase 1 (SRD5a1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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