Bioactivity
(Taste receptor type 1 member 3 (TAS1R3), responsible for mediating taste perception, is a G protein-coupled receptor (GPCR) expressed in gustatory cells located within the taste buds on the tongue. It can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TAS1R3 and recombinant mouse TAS1R1. Briefly, biotin-linked TAS1R3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TAS1R1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human TAS1R3 and recombinant mouse TAS1R1 was shown in Figure 1, the EC50 for this effect is 0.31 ug/mL.)
Taste Receptor Type 1 Member 3 (TAS1R3) Active Protein | TAS1R3 active protein
Active Taste Receptor Type 1 Member 3 (TAS1R3)
Gene Names
TAS1R3; T1R3
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Taste Receptor Type 1 Member 3 (TAS1R3); N/A; Active Taste Receptor Type 1 Member 3 (TAS1R3); Taste Receptor Type 1 Member 3; T1R3; Sweet taste receptor T1R34; TAS1R3 active protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Ala21~Leu570
Applicable Applications for TAS1R3 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
7
Research Area
CD & Adhesion molecule
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Taste receptor type 1 member 3 (TAS1R3), responsible for mediating taste perception, is a G protein-coupled receptor (GPCR) expressed in gustatory cells located within the taste buds on the tongue. It can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TAS1R3 and recombinant mouse TAS1R1. Briefly, biotin-linked TAS1R3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TAS1R1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human TAS1R3 and recombinant mouse TAS1R1 was shown in Figure 1, the EC50 for this effect is 0.31 ug/mL.)
Bioactivity
(Taste receptor type 1 member 3 (TAS1R3), responsible for mediating taste perception, is a G protein-coupled receptor (GPCR) expressed in gustatory cells located within the taste buds on the tongue. It can form a heterodimeric receptor with TAS1R1 to elicit the umami taste response, or it can bind with TAS1R2 to form a receptor for the sweet taste response, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TAS1R3 and recombinant mouse TAS1R1. Briefly, biotin-linked TAS1R3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TAS1R1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human TAS1R3 and recombinant mouse TAS1R1 was shown in Figure 1, the EC50 for this effect is 0.31 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for TAS1R3 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,386 Da
NCBI Official Full Name
taste receptor type 1 member 3
NCBI Official Synonym Full Names
taste receptor, type 1, member 3
NCBI Official Symbol
TAS1R3
NCBI Official Synonym Symbols
T1R3
NCBI Protein Information
taste receptor type 1 member 3; sweet taste receptor T1R3
UniProt Protein Name
Taste receptor type 1 member 3
UniProt Gene Name
TAS1R3
UniProt Synonym Gene Names
T1R3; TR3
UniProt Entry Name
TS1R3_HUMAN
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Product Notes
The TAS1R3 tas1r3 (Catalog #AAA161923) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Taste Receptor Type 1 Member 3 (TAS1R3) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the TAS1R3 tas1r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala21~Leu5 70. It is sometimes possible for the material contained within the vial of "Taste Receptor Type 1 Member 3 (TAS1R3), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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