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WB (Western Blot)
(Figure 4. Western Blot Sample: Recombinant TGF-Beta1, Human; Antibody: Rabbit Anti8Human TGF-Beta1 Ab)
Transforming Growth Factor Beta 1 (TGFb1) Active Protein | TGFb1 active protein
Active Transforming Growth Factor Beta 1 (TGFb1)
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Applications
Activity Assay
Purity
>98%
Synonyms
Transforming Growth Factor Beta 1 (TGFb1); N/A; Active Transforming Growth Factor Beta 1 (TGFb1); TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide; TGFb1 active protein
Host
E. coli
Purity/Purification
>98%
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Concentration
Original Concentration: 400ug/mL (varies by lot)
Applicable Applications for TGFb1 active protein
Activity Assay
Source
Prokaryotic expression.
Residues
Ala279~Ser390
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per ug (determined by the LAL method)
Predicted isoelectric point
8.4
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Figure 4. Western Blot Sample: Recombinant TGF-Beta1, Human; Antibody: Rabbit Anti8Human TGF-Beta1 Ab)
WB (Western Blot)
(Figure 4. Western Blot Sample: Recombinant TGF-Beta1, Human; Antibody: Rabbit Anti8Human TGF-Beta1 Ab)
SDS_PAGE
(Figure 3. SDS-PAGE Sample: Active recombinant TGF-Beta1, Human)
SDS_PAGE
(Figure 3. SDS-PAGE Sample: Active recombinant TGF-Beta1, Human)
Identification
(Figure 2. Gene Sequencing (extract))
Identification
(Figure 2. Gene Sequencing (extract))
ELISA
(TGF-beta1 (Transforming growth factor beta 1) is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF beta 1 has been shown to interact with TGF beta receptor 1, Decorin, LTBP1 and so on. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human TGF-Beta1 with recombinant human LTBP1. Briefly, TGF-Beta1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LTBP1"coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti"LTBP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15"25 minutes at 37°C. Finally, add 50BL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-Beta1 with LTBP1 was shown in Figure 1 and this effect was in a dose dependent manner.Figure 1. The binding activity of TGF-Beta1 with LTBP1.)
ELISA
(TGF-beta1 (Transforming growth factor beta 1) is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF beta 1 has been shown to interact with TGF beta receptor 1, Decorin, LTBP1 and so on. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human TGF-Beta1 with recombinant human LTBP1. Briefly, TGF-Beta1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LTBP1"coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti"LTBP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15"25 minutes at 37°C. Finally, add 50BL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-Beta1 with LTBP1 was shown in Figure 1 and this effect was in a dose dependent manner.Figure 1. The binding activity of TGF-Beta1 with LTBP1.)
Sequence
Sequence
Product Categories/Family for TGFb1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 14.1kDa
Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
transforming growth factor beta-1 preproprotein
NCBI Official Synonym Full Names
transforming growth factor beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
NCBI Protein Information
transforming growth factor beta-1
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP
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Product Notes
The TGFb1 tgfb1 (Catalog #AAA148233) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Transforming Growth Factor Beta 1 (TGFb1) can be used in a range of immunoassay formats including, but not limited to, Activity Assay. Researchers should empirically determine the suitability of the TGFb1 tgfb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor Beta 1 (TGFb1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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