Tumor Necrosis Factor Beta (TNFb) Active Protein | TNFb active protein

Active Tumor Necrosis Factor Beta (TNFb)

Cat. Num. AAA146616

• Gene Names: LTA; LT; TNFB; TNFSF1; TNLG1E
• Applications: Activity Assay, Cell Culture
• Purity: > 97%
• Synonyms: Tumor Necrosis Factor Beta (TNFb); N/A; Active Tumor Necrosis Factor Beta (TNFb); LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha; TNFb active protein

• Host: E Coli
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 205

• Applicable Applications for TNFb active protein: Activity Assay, Cell Culture
• Fragment: Pro35~Leu205
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Human
• Isoelectric Point: 9.3
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

Application Data

SDS-PAGE

Activity

(TNF-beta, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-beta are very similar to TNF-alpha, due to the similarity of molecular structure and the receptors. As reported, TNF-beta could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1beta in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-beta (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1beta was detected in the cell supernatant by ELISA .Cell necrosis after incubation with TNF-beta(10ng/mL) for 72h was shown in Figure 1.)

Product Categories/Family for TNFb active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 6806893
• NCBI GeneID: 4049
• NCBI Accession #: NP_000586.2
• NCBI GenBank Nucleotide #: NP_000586.2
• UniProt Accession #: P01374; Q8N4C3; Q9UKS8
• Molecular Weight: 22.2kDa; 23kDa
• NCBI Official Full Name: lymphotoxin-alpha
• NCBI Official Synonym Full Names: lymphotoxin alpha
• NCBI Official Symbol: LTA
• NCBI Official Synonym Symbols: LT; TNFB; TNFSF1; TNLG1E
• NCBI Protein Information: lymphotoxin-alpha
• UniProt Protein Name: Lymphotoxin-alpha
• UniProt Gene Name: LTA
• UniProt Synonym Gene Names: TNFB; TNFSF1; LT-alpha