Jun Proto-Oncogene Active Protein | C-JUN active protein
Recombinant Human Jun Proto-Oncogene
Gene Names
JUN; AP1; AP-1; c-Jun
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Jun Proto-Oncogene; N/A; Recombinant Human Jun Proto-Oncogene; C-JUN Human; Jun Proto-Oncogene Human Recombinant; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; c-Jun; C-JUN active protein
Host
E Coli
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
C-JUN is supplied as lyophilized powder containing no additives.
Sequence Length
331
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml.
Biological Activity
C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 1-2 weeks.
Store, frozen at -20 degree C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Store, frozen at -20 degree C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Related Product Information for C-JUN active protein
C-JUN amino acids 1-81 produced in E Coli, is a non-glycosylated, polypeptide chain having a molecular mass of 52 kDa. C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques.
Product Categories/Family for C-JUN active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
jun proto-oncogene
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; AP-1; c-Jun
NCBI Protein Information
transcription factor AP-1; Jun activation domain binding protein; activator protein 1; enhancer-binding protein AP1; jun oncogene; p39; proto-oncogene c-Jun; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog
UniProt Protein Name
Transcription factor AP-1
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1
UniProt Entry Name
JUN_HUMAN
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Product Notes
The C-JUN jun (Catalog #AAA38414) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Jun Proto-Oncogene, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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