SDS-PAGE
(Sample: Active recombinant SOD4, Mouse)
Superoxide Dismutase Copper Chaperone Active Protein | CCS active protein
Active Superoxide Dismutase Copper Chaperone
Gene Names
Ccs; Ccsd
Reactivity
Mus musculus (Mouse)
Applications
Activity Assay, Cell Culture
Purity
>95%
Synonyms
Superoxide Dismutase Copper Chaperone; N/A; Active Superoxide Dismutase Copper Chaperone; CCS; Copper Chaperone For Superoxide Dismutase4; CCS active protein
Host
E. coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
>95%
Form/Format
PBS, pH7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Concentration
Original Concentration: 400ug/mL (varies by lot)
Applicable Applications for CCS active protein
Activity Assay, Cell Culture
Source
Prokaryotic expression.
Residues
Ala17~Pro270
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
6.3
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
(Sample: Active recombinant SOD4, Mouse)
SDS-PAGE
(Sample: Active recombinant SOD4, Mouse)
Bioactivity
(Superoxide dismutase copper chaperone (SOD4) is an enzyme that in humans is encoded by the CCS gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant mouse SOD4. The reaction was performed in adding 8 ul 5 mmol/L pyrogallol to 200 ul 50mmol/L Tris-HCl, rapidly mixing at 25 degree C, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant mouse SOD4 were added into 200 ul 50 mmol/L Tris-HCl, incubated for 20 min at 25 degree C, then adding 8 ul 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant mouse SOD4 is 66.88 U/mg.)
Bioactivity
(Superoxide dismutase copper chaperone (SOD4) is an enzyme that in humans is encoded by the CCS gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant mouse SOD4. The reaction was performed in adding 8 ul 5 mmol/L pyrogallol to 200 ul 50mmol/L Tris-HCl, rapidly mixing at 25 degree C, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant mouse SOD4 were added into 200 ul 50 mmol/L Tris-HCl, incubated for 20 min at 25 degree C, then adding 8 ul 5 mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes. Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant mouse SOD4 is 66.88 U/mg.)
Sequence
Sequence
Product Categories/Family for CCS active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 30.6kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
copper chaperone for superoxide dismutase
NCBI Official Synonym Full Names
copper chaperone for superoxide dismutase
NCBI Official Symbol
Ccs
NCBI Official Synonym Symbols
Ccsd
NCBI Protein Information
copper chaperone for superoxide dismutase; superoxide dismutase copper chaperone
UniProt Protein Name
Copper chaperone for superoxide dismutase
UniProt Gene Name
Ccs
UniProt Synonym Gene Names
Ccsd
UniProt Entry Name
CCS_MOUSE
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Product Notes
The CCS ccs (Catalog #AAA161750) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Superoxide Dismutase Copper Chaperone reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Superoxide Dismutase Copper Chaperone can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the CCS ccs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase Copper Chaperone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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