Specific Activity
(Sample Activity Plot.)
COVID 19 PLpro Coronavirus Active Protein | COVID-19 active protein
2019-nCoV PLpro, Active
Synonyms
COVID 19 PLpro Coronavirus; N/A; 2019-nCoV PLpro, Active; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; PLpro; COVID-19 active protein
Host
E Coli
Form/Format
50mM sodium phosphate, pH7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol
Species
SARS-CoV-2
Tag
HIS
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Specific Activity
(Sample Activity Plot.)
Specific Activity
(Sample Activity Plot.)
Application Data
(PLpro inhibition profile in the presence of various concentrations of PR-619, a broad spectrum and reversible DUB inhibitor.)
Application Data
(PLpro inhibition profile in the presence of various concentrations of PR-619, a broad spectrum and reversible DUB inhibitor.)
Related Product Information for COVID-19 active protein
Recombinant 2019-nCoV PLpro/papain-like protease, part of a large replicase polyprotein 1ab (E1564-Y1882), was expressed in E Coli cells with an N-terminal His tag.
Overview: 2019-nCoV papain-like protease (PLpro), also known as SARS-CoV-2 PLpro, is a virally encoded cysteine protease that acts on three cleavage sites of the viral polyprotein to release mature non-structural proteins (nsp) 1, 2 and 3, a process that is essential for viral replication. In addition, PLpro possesses deubiquitinating and deISGylating activities, thus negatively regulating the ubiquitin and ISG15-dependent host immune response during viral infection. PLpro also participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.
Overview: 2019-nCoV papain-like protease (PLpro), also known as SARS-CoV-2 PLpro, is a virally encoded cysteine protease that acts on three cleavage sites of the viral polyprotein to release mature non-structural proteins (nsp) 1, 2 and 3, a process that is essential for viral replication. In addition, PLpro possesses deubiquitinating and deISGylating activities, thus negatively regulating the ubiquitin and ISG15-dependent host immune response during viral infection. PLpro also participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.
Product Categories/Family for COVID-19 active protein
References
1. Wu F. et al: A new Corona virus associated with human respiratory disease in China. Nature 579: 265-269, 2020
2. Báez-Santos Y. M. et al: The SARS-Corona virus papain-like protease: structure, function and inhibition by designed antiviral compounds. Antiviral Res. 115: 21-38, 2015
3. Lindner H. A. et al: The papain-like protease from the severe acute respiratory syndrome Corona virus is a deubiquitinating enzyme. J Virol. 79:15199-15208, 2005
2. Báez-Santos Y. M. et al: The SARS-Corona virus papain-like protease: structure, function and inhibition by designed antiviral compounds. Antiviral Res. 115: 21-38, 2015
3. Lindner H. A. et al: The papain-like protease from the severe acute respiratory syndrome Corona virus is a deubiquitinating enzyme. J Virol. 79:15199-15208, 2005
NCBI and Uniprot Product Information
Molecular Weight
38kDa
Similar Products
Product Notes
The COVID-19 (Catalog #AAA73170) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "COVID 19 PLpro Coronavirus, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.