Standard Curve (Sample)
E coli O157 ELISA Kit | O157 elisa kit
E coli O157 ELISA Kit
Reactivity
E coli
Synonyms
O157; N/A; E coli O157 ELISA Kit; O157 elisa kit
Reactivity
E coli
Detection Method
Colorimetric
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for O157 elisa kit
Background: E. coli is one of the many groups of bacteria that live in the intestines of healthy humans and most warm-blooded animals. E. coli's serotyping is based on the flagellar (H) and polysaccharide (O) antigens. Shiga toxin-producing E.coli strains are major foodborne pathogens and have caused a large number of human illnesses. E.coli O157:H7 is the most well-known serotype and it can cause diarrhea, hemorrhagic colitis, and in more severe cases hemolytic uremic syndrome. Most E.coli O157:H7 infection outbreaks have been linked to the consumption of ground beef. The E. coli O157 ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the E. coli O157. The ELISA antibodies specifically recognize the E.coli O157 strain, will not react with other E. coli strains with different O antigens and other types of bacteria such as Shigella sonnei, Pseudomonas aeruginosa and Salmonella flexneri. The ELISA kit has a detection sensitivity limit of 1.2 x 104 CFU equivalents/mL E. coli O157. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and food test samples.
Principle of the Assay: An anti-E.coli O157 monoclonal antibody is adsorbed onto a microtiter plate. E.coli O157 present in the pre-enriched sample or standard binds to the antibody adsorbed on the plate; an FITC-conjugated anti-E.coli O157 monoclonal antibody is added and binds to the E.coli O157 captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-E.coli O157 monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and a substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of E.coli O157 present in the sample. The reaction is terminated by addition of Stop Solution and the absorbance is measured at 450 nm. A standard curve is prepared from the provided heat killed E.coli O157 standard and the sample E.coli O157 amount is then determined.
Principle of the Assay: An anti-E.coli O157 monoclonal antibody is adsorbed onto a microtiter plate. E.coli O157 present in the pre-enriched sample or standard binds to the antibody adsorbed on the plate; an FITC-conjugated anti-E.coli O157 monoclonal antibody is added and binds to the E.coli O157 captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-E.coli O157 monoclonal antibody. Unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and a substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of E.coli O157 present in the sample. The reaction is terminated by addition of Stop Solution and the absorbance is measured at 450 nm. A standard curve is prepared from the provided heat killed E.coli O157 standard and the sample E.coli O157 amount is then determined.
Product Categories/Family for O157 elisa kit
Similar Products
Product Notes
The E coli O157 (Catalog #AAA44700) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA44700 ELISA Kit recognizes E coli O157. It is sometimes possible for the material contained within the vial of "O157, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.