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product-image-AAA23885_APP.jpg Application Data (In order to compare the ability to inhibit ribonuclease activity, rat RNA was pre-mixed with RNaseOFF (Left image) and the leading competitor's RNase (right image) in a 20ul reaction. Increasing amoutn of RNase A enzyme ranging from 5ng to 50ng per reaction was added to into each reaction mixture. The reactions were incubated at 37°C for 15 minutes and loaded onto a 2% gel to viasualize the degree of RNA degradation. At 15ng/rxn of of RNase A, both 285 and 185 ribosomal RNA bands are clearly visible in RNaseOff Reaction (left image). However , the 285 band is degraded in the competitor's reaction (right image).)

ribonuclease enzyme

RNaseOFF Ribonuclease Inhibitor

Synonyms
Ribonuclease; N/A; RNaseOFF Ribonuclease Inhibitor; ribonuclease enzyme
Ordering
For Research Use Only!
Host
E. coli
Sequence Length
163
Application Notes
Always keep RNaseOFF on ice during reaction set-up. It is recommended to set-up all RNA-related or RNase-sensitive work under conditions where RNase contamination has been eliminated. The use of “clean” pipettors designated for PCR and aerosol-resistant barrier tips are recommended.

RNaseOFF should be added to the reaction tube prior to the addition of other components (including the sample) to ensure RNase inhibitory activity. Prevent inactivation of RNaseOFF and release of active ribonuclease by avoiding temperatures greater than 50°C, and high concentrations of urea or other denaturing agents.
Notes
One unit is defined as the amount of RNaseOFF Ribonuclease Inhibitor that is required to inhibit the activity of 5 ng of RNase A by 50%. Always keep RNaseOFF on ice during reaction set-up. It is recommended to setup all RNA-related/RNase-sensitive work under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% DEPC. Wearing gloves is highly recommended while working with RNA.
Preparation and Storage
Store all components at -20°C.

Application Data

(In order to compare the ability to inhibit ribonuclease activity, rat RNA was pre-mixed with RNaseOFF (Left image) and the leading competitor's RNase (right image) in a 20ul reaction. Increasing amoutn of RNase A enzyme ranging from 5ng to 50ng per reaction was added to into each reaction mixture. The reactions were incubated at 37°C for 15 minutes and loaded onto a 2% gel to viasualize the degree of RNA degradation. At 15ng/rxn of of RNase A, both 285 and 185 ribosomal RNA bands are clearly visible in RNaseOff Reaction (left image). However , the 285 band is degraded in the competitor's reaction (right image).)

product-image-AAA23885_APP.jpg Application Data (In order to compare the ability to inhibit ribonuclease activity, rat RNA was pre-mixed with RNaseOFF (Left image) and the leading competitor's RNase (right image) in a 20ul reaction. Increasing amoutn of RNase A enzyme ranging from 5ng to 50ng per reaction was added to into each reaction mixture. The reactions were incubated at 37°C for 15 minutes and loaded onto a 2% gel to viasualize the degree of RNA degradation. At 15ng/rxn of of RNase A, both 285 and 185 ribosomal RNA bands are clearly visible in RNaseOff Reaction (left image). However , the 285 band is degraded in the competitor's reaction (right image).)
Related Product Information for ribonuclease enzyme
RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation over the highly oxidation-sensitive human RNase inhibitor. RNaseOFF Ribonuclease Inhibitor is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
ribonuclease
UniProt Protein Name
Ribonuclease
UniProt Gene Name
ribonuclease

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Product Notes

The Ribonuclease ribonuclease (Catalog #AAA23885) is an Enzyme produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. Always keep RNaseOFF on ice during reaction set-up. It is recommended to set-up all RNA-related or RNase-sensitive work under conditions where RNase contamination has been eliminated. The use of “clean” pipettors designated for PCR and aerosol-resistant barrier tips are recommended. RNaseOFF should be added to the reaction tube prior to the addition of other components (including the sample) to ensure RNase inhibitory activity. Prevent inactivation of RNaseOFF and release of active ribonuclease by avoiding temperatures greater than 50°C, and high concentrations of urea or other denaturing agents. Researchers should empirically determine the suitability of the Ribonuclease ribonuclease for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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