Growth Arrest Specific Protein 6 (GAS6) Active Protein | GAS6 active protein

Active Growth Arrest Specific Protein 6 (GAS6)

Cat. Num. AAA21055

• Gene Names: GAS6; AXSF; AXLLG
• Reactivity: Homo sapiens (Human)
• Applications: Cell culture; Activity Assays.
• Purity: >92%
• Synonyms: Growth Arrest Specific Protein 6 (GAS6); N/A; Active Growth Arrest Specific Protein 6 (GAS6); AXLLG; AXSF; AXL Stimulatory Factor; AXL receptor tyrosine kinase ligand; GAS6 active protein

• Host: E. coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: >92%
• Form/Format: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 800ug/mL (varies by lot)

• Applicable Applications for GAS6 active protein: Cell culture; Activity Assays.
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Leu136~Phe311
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Predicted isoelectric point: 5.6
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved togive the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Sample: Recombinant GAS6, Human;Antibody: Rabbit Anti-Human GAS6 Ab)

SDS-PAGE

(SDS-PAGESample: Active recombinant GAS6, Human)

Identification

(Gene Sequencing (extract))

Application Data

(The dose-effect curve of GAS6 was shown in Figure 2. It was obvious that GAS6 significantly promoted cell proliferation of DU145 cells. The ED50 for this effect is typically 0.77~38.08ng/mL.)

Activity

(Growth arrest-specific 6, also known as GAS6, is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation. It has been reported that both PC-3 and DU 145 human prostate cancer cell lines are stimulated to proliferate by Gas6, however, this proliferative response strictly correlates with the expression of the Axl receptor, being higher in DU 145 cells. To test the proliferative effect of Gas6, DU 145 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of GAS6. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.Cell proliferation of DU145 cells after incubation with GAS6 for 72h observed by inverted microscope was shown in Figure 1.The dose-effect curve of GAS6 was shown in Figure 2. It was obvious that GAS6 significantly promoted cell proliferation of DU145 cells. The ED50 for this effect is typically 0.77~38.08ng/mL.)

Sequence Information

Product Categories/Family for GAS6 active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4557617
• NCBI GeneID: 2621
• NCBI Accession #: NP_000811.1
• NCBI GenBank Nucleotide #: NM_000820.3
• UniProt Accession #: Q14393; Q6IMN1; Q7Z7N3; B3KRQ7; B3KVL4; E9PBL7
• Molecular Weight: Predicted Molecular Mass: 20.4kDa; Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: growth arrest-specific protein 6
• NCBI Official Synonym Full Names: growth arrest specific 6
• NCBI Official Symbol: GAS6
• NCBI Official Synonym Symbols: AXSF; AXLLG
• NCBI Protein Information: growth arrest-specific protein 6
• UniProt Protein Name: Growth arrest-specific protein 6
• UniProt Gene Name: GAS6
• UniProt Synonym Gene Names: AXLLG; GAS-6