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WB (Western Blot)
(Sample: Recombinant ITIH4, Human;Antibody: Rabbit Anti-Human ITIH4 Ab)
Inter Alpha-Globulin Inhibitor H4 (ITIH4) Active Protein | ITIH4 active protein
Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)
Gene Names
ITIH4; H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>98%
Synonyms
Inter Alpha-Globulin Inhibitor H4 (ITIH4); N/A; Active Inter Alpha-Globulin Inhibitor H4 (ITIH4); IHRP; PK120; H4P; ITIHL1; Plasma Kallikrein-Sensitive Glycoprotein; Plasma kallikrein sensitive glycoprotein 120; Inter-alpha-trypsin inhibitor family heavy chain-related protein; ITIH4 active protein
Host
E. coli
Reactivity
Homo sapiens (Human)
Purity/Purification
>98%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 1200ug/mL (varies by lot)
Applicable Applications for ITIH4 active protein
Activity Assay, Cell Culture
Source
Prokaryotic expression.
Residues
Ala683~Val928
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
6.9
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Sample: Recombinant ITIH4, Human;Antibody: Rabbit Anti-Human ITIH4 Ab)
WB (Western Blot)
(Sample: Recombinant ITIH4, Human;Antibody: Rabbit Anti-Human ITIH4 Ab)
SDS-PAGE
(Sample: Active recombinant ITIH4, Human)
SDS-PAGE
(Sample: Active recombinant ITIH4, Human)
Bioactivity
(Inter Alpha-Globulin Inhibitor H4 (ITIH4) is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. ITIH4 has been detected only in liver, and it seems to be upregulated during surgical trauma. It may also play a role in liver development or regeneration. Besides, Growth Factor Receptor Bound Protein 2 (Grb2) has been identified as an interactor of ITIH4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ITIH4 and recombinant human Grb2. Briefly, ITIH4 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Grb2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ITIH4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ITIH4 and Grb2 was shown in Figure 1, and this effect was in a dose dependent manner.The binding activity of ITIH4with Grb2.)
Bioactivity
(Inter Alpha-Globulin Inhibitor H4 (ITIH4) is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. ITIH4 has been detected only in liver, and it seems to be upregulated during surgical trauma. It may also play a role in liver development or regeneration. Besides, Growth Factor Receptor Bound Protein 2 (Grb2) has been identified as an interactor of ITIH4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ITIH4 and recombinant human Grb2. Briefly, ITIH4 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to Grb2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ITIH4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ITIH4 and Grb2 was shown in Figure 1, and this effect was in a dose dependent manner.The binding activity of ITIH4with Grb2.)
Sequence
Sequence
Product Categories/Family for ITIH4 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 28.9kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
ITIH4 protein
NCBI Official Synonym Full Names
inter-alpha-trypsin inhibitor heavy chain family member 4
NCBI Official Symbol
ITIH4
NCBI Official Synonym Symbols
H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
NCBI Protein Information
inter-alpha-trypsin inhibitor heavy chain H4
UniProt Protein Name
Inter-alpha-trypsin inhibitor heavy chain H4
UniProt Gene Name
ITIH4
UniProt Synonym Gene Names
IHRP; ITIHL1; PK120; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; IHRP; Gp120; PK-120
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Product Notes
The ITIH4 itih4 (Catalog #AAA149264) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Inter Alpha-Globulin Inhibitor H4 (ITIH4) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Inter Alpha-Globulin Inhibitor H4 (ITIH4) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the ITIH4 itih4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Inter Alpha-Globulin Inhibitor H4 (ITIH4), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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