<
WB (Western Blot)
(Sample: Recombinant IL10Rb, Human;Antibody: Rabbit Anti-Human IL10Rb Ab)
Interleukin 10 Receptor Beta (IL10Rb) Active Protein | IL10Rb active protein
Active Interleukin 10 Receptor Beta (IL10Rb)
Gene Names
IL10RB; CRFB4; CRF2-4; D21S58; D21S66; CDW210B; IL-10R2
Reactivity
Homo sapiens (Human)
Applications
Invitro Assay, Activity Assay, Cell Culture
Purity
>98%
Synonyms
Interleukin 10 Receptor Beta (IL10Rb); N/A; Active Interleukin 10 Receptor Beta (IL10Rb); CDW210b; CD210-B; IL10R-B; IL10-RB; CRF2-4; CRFB4; IL-10R2; Interleukin-10 receptor subunit 2; IL10Rb active protein
Host
E. coli
Reactivity
Homo sapiens (Human)
Purity/Purification
>98%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 80ug/mL (varies by lot)
Applicable Applications for IL10Rb active protein
Invitro Assay, Activity Assay, Cell Culture
Source
Prokaryotic expression.
Residues
Leu87~Ala243
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
5.0
Usage
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
WB (Western Blot)
(Sample: Recombinant IL10Rb, Human;Antibody: Rabbit Anti-Human IL10Rb Ab)
WB (Western Blot)
(Sample: Recombinant IL10Rb, Human;Antibody: Rabbit Anti-Human IL10Rb Ab)
Gene Sequencing (extract)
Gene Sequencing (extract)
SDS-PAGE
(Sample: Active recombinant IL10Rb, Human)
SDS-PAGE
(Sample: Active recombinant IL10Rb, Human)
Bioactivity
(IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was shown in Figure 1, and this effect was in a dose dependent manner.)
Bioactivity
(IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was shown in Figure 1, and this effect was in a dose dependent manner.)
Sequence
Sequence
Product Categories/Family for IL10Rb active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 19.5kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
interleukin-10 receptor subunit beta
NCBI Official Synonym Full Names
interleukin 10 receptor subunit beta
NCBI Official Symbol
IL10RB
NCBI Official Synonym Symbols
CRFB4; CRF2-4; D21S58; D21S66; CDW210B; IL-10R2
NCBI Protein Information
interleukin-10 receptor subunit beta
UniProt Protein Name
Interleukin-10 receptor subunit beta
UniProt Gene Name
IL10RB
UniProt Synonym Gene Names
CRFB4; D21S58; D21S66; IL-10 receptor subunit beta; IL-10R subunit beta; IL-10RB; CRF2-4; IL-10R subunit 2; IL-10R2
Similar Products
Product Notes
The IL10Rb il10rb (Catalog #AAA148125) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Interleukin 10 Receptor Beta (IL10Rb) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 10 Receptor Beta (IL10Rb) can be used in a range of immunoassay formats including, but not limited to, Invitro Assay, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the IL10Rb il10rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 10 Receptor Beta (IL10Rb), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.