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WB (Western Blot)
(Sample: Recombinant IL35, Human;Antibody: Rabbit Anti-Human IL35 Ab)
Interleukin 35 (IL35) Active Protein | IL35 active protein
Active Interleukin 35 (IL35)
Gene Names
IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays.
Purity
>98%
Synonyms
Interleukin 35 (IL35); N/A; Active Interleukin 35 (IL35); IL35 active protein
Host
E. coli
Reactivity
Homo sapiens (Human)
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl, 5%Trehalose.
Applicable Applications for IL35 active protein
Cell culture; Activity Assays; In vivo assays.
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
The IL35 complex composed of IL27B subunit (Arg21~Lys229) and the mature form of human IL12A (Arg23~Ser219) linked by a polypeptide linker (GGGGS)3
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
8.7
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Sample: Recombinant IL35, Human;Antibody: Rabbit Anti-Human IL35 Ab)
WB (Western Blot)
(Sample: Recombinant IL35, Human;Antibody: Rabbit Anti-Human IL35 Ab)
SDS-PAGE
(Sample: Active recombinant IL35, Human)
SDS-PAGE
(Sample: Active recombinant IL35, Human)
Identification
(Gene Sequencing (extract))
Identification
(Gene Sequencing (extract))
Application Data
Application Data
Activity
(IL35 (Interleukin 35) is an IL-12 family cytokine, which is a dimeric protein composed of IL-12alpha and IL-27beta chains. IL35 is thought to mediate the immune inhibitory function of regulatory T cells and has been proven to promotes pancreas cancer growth through enhancement of proliferation and inhibition of apoptosis. Thus, proliferation assay of IL35 was conducted using PANC-1 cells. Briefly, PANC-1 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of recombinant human IL35. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of PANC-1 cells after incubation with IIL35 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with human recombinant IL35 for 48h. The result was shown in Figure 2. It was obvious that IL35 significantly increased cell viability of PANC-1 cells.)
Activity
(IL35 (Interleukin 35) is an IL-12 family cytokine, which is a dimeric protein composed of IL-12alpha and IL-27beta chains. IL35 is thought to mediate the immune inhibitory function of regulatory T cells and has been proven to promotes pancreas cancer growth through enhancement of proliferation and inhibition of apoptosis. Thus, proliferation assay of IL35 was conducted using PANC-1 cells. Briefly, PANC-1 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of recombinant human IL35. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of PANC-1 cells after incubation with IIL35 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with human recombinant IL35 for 48h. The result was shown in Figure 2. It was obvious that IL35 significantly increased cell viability of PANC-1 cells.)
Sequence Information
Sequence Information
Product Categories/Family for IL35 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 51.7kDa
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
interleukin-12 subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 12A
NCBI Official Symbol
IL12A
NCBI Official Synonym Symbols
P35; CLMF; NFSK; NKSF1; IL-12A
NCBI Protein Information
interleukin-12 subunit alpha
UniProt Protein Name
Interleukin-12 subunit alpha
UniProt Gene Name
IL12A
UniProt Synonym Gene Names
NKSF1; IL-12A; CLMF p35; NKSF1
Similar Products
Product Notes
The IL35 il12a (Catalog #AAA21102) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Interleukin 35 (IL35) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 35 (IL35) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the IL35 il12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 35 (IL35), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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