Myxovirus Resistance 1 (MX1) Active Protein | MX1 active protein

Active Myxovirus Resistance 1 (MX1)

Cat. Num. AAA149266

• Gene Names: MX1; MX; MxA; IFI78; IFI-78K
• Reactivity: Homo sapiens (Human)
• Applications: Activity Assay, Cell Culture
• Purity: >98%
• Synonyms: Myxovirus Resistance 1 (MX1); N/A; Active Myxovirus Resistance 1 (MX1); MX1 active protein

• Host: E. coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: >98%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl and 5% trehalose.

• Applicable Applications for MX1 active protein: Activity Assay, Cell Culture
• Source: Prokaryotic expression.
• Residues: Ser80~Leu342
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted isoelectric point: 5.8
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give theactive form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Figure 4. Western BlotSample: Recombinant MX1, Human;Antibody: Rabbit Anti-Human MX1 Ab)

SDS_PAGE

(Figure 3. SDS-PAGESample: Active recombinant MX1, Human)

Identification

(Figure 2. Gene Sequencing (extract))

Bioactivity

(Figure.1 The binding activity of MX1 with TUBb.)

Sequence

Product Categories/Family for MX1 active protein

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 251757499
• NCBI GeneID: 4599
• Molecular Weight: Predicted Molecular Mass: 30.3kDa; Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: Interferon-induced GTP-binding protein Mx1
• NCBI Official Synonym Full Names: MX dynamin like GTPase 1
• NCBI Official Symbol: MX1
• NCBI Official Synonym Symbols: MX; MxA; IFI78; IFI-78K
• NCBI Protein Information: interferon-induced GTP-binding protein Mx1
• UniProt Protein Name: Interferon-induced GTP-binding protein Mx1
• UniProt Gene Name: MX1
• UniProt Synonym Gene Names: IFI-78K
• UniProt Entry Name: MX1_HUMAN