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WB (Western Blot)
(Sample: Recombinant TRAIL, Human;Antibody: Rabbit Anti-Human TRAIL Ab)
Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Active Protein | TRAIL active protein
Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays; In vivo assays
Purity
>95%
Synonyms
Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL); N/A; Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL); TRAIL active protein
Host
E. coli
Reactivity
Homo sapiens (Human)
Purity/Purification
>95%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose
Concentration
Original Concentration: 450ug/mL (varies by lot)
Applicable Applications for TRAIL active protein
Cell culture; Activity Assays; In vivo assays
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression.
Residues
Val114~Gly281
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
8.6
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Sample: Recombinant TRAIL, Human;Antibody: Rabbit Anti-Human TRAIL Ab)
WB (Western Blot)
(Sample: Recombinant TRAIL, Human;Antibody: Rabbit Anti-Human TRAIL Ab)
SDS-PAGE
(Sample: Active recombinant TRAIL, Human)
SDS-PAGE
(Sample: Active recombinant TRAIL, Human)
Gene Sequencing (extract)
Gene Sequencing (extract)
Activity Data
(Protein TRAIL (TNF-Related Apoptosis-Inducing Ligand) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL induces apoptosis in transformed and tumor cells. This protein binds to several members of TNF receptor superfamily including TNFRSF10B/TRAILR2. Thus a binding ELISA assay was conducted to detect the interaction of TRAIL and TNFRSF10B. Briefly, recombinant human TRAIL were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFRSF10B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TRAIL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TRAIL and TNFRSF10B was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(Protein TRAIL (TNF-Related Apoptosis-Inducing Ligand) is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL induces apoptosis in transformed and tumor cells. This protein binds to several members of TNF receptor superfamily including TNFRSF10B/TRAILR2. Thus a binding ELISA assay was conducted to detect the interaction of TRAIL and TNFRSF10B. Briefly, recombinant human TRAIL were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TNFRSF10B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TRAIL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TRAIL and TNFRSF10B was shown in Figure 1, and this effect was in a dose dependent manner.)
Sequence Information
Sequence Information
Product Categories/Family for TRAIL active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 19.6kDa
Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 19kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 10 isoform 1
NCBI Official Synonym Full Names
TNF superfamily member 10
NCBI Official Symbol
TNFSF10
NCBI Official Synonym Symbols
TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
NCBI Protein Information
tumor necrosis factor ligand superfamily member 10
UniProt Protein Name
TNFSF10 protein
UniProt Gene Name
TNFSF10
Similar Products
Product Notes
The TRAIL tnfsf10 (Catalog #AAA21106) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the TRAIL tnfsf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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