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Application Data
Cytochrome P450 1A2 (CYP1A2) Active Protein | CYP1A2 active protein
Active Cytochrome P450 1A2 (CYP1A2)
Gene Names
CYP1A2; CP12; CYPIA2; P3-450; P450(PA)
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 95%
Synonyms
Cytochrome P450 1A2 (CYP1A2); N/A; Active Cytochrome P450 1A2 (CYP1A2); CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2; CYP1A2 active protein
Host
E Coli
Reactivity
Human
Purity/Purification
> 95%
Form/Format
Freeze-dried powder
Sequence Length
516
Applicable Applications for CYP1A2 active protein
Activity Assay, Cell Culture
Tag
N-terminal His and GST Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Ala2~Ser231
Isoelectric Point
8
Application Data
Application Data
Application Data
Application Data
SDS-PAGE
SDS-PAGE
Activity
(CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity
(CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Product Categories/Family for CYP1A2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Weight: 55.2kDa
Accurate Molecular Weight: 55kDa
Accurate Molecular Weight: 55kDa
NCBI Official Full Name
cytochrome P450 1A2
NCBI Official Synonym Full Names
cytochrome P450 family 1 subfamily A member 2
NCBI Official Symbol
CYP1A2
NCBI Official Synonym Symbols
CP12; CYPIA2; P3-450; P450(PA)
NCBI Protein Information
cytochrome P450 1A2
UniProt Protein Name
Cytochrome P450 1A2
UniProt Gene Name
CYP1A2
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Product Notes
The CYP1A2 cyp1a2 (Catalog #AAA148207) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Active Cytochrome P450 1A2 (CYP1A2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 1A2 (CYP1A2) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the CYP1A2 cyp1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 1A2 (CYP1A2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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