SDS-PAGE
(SDS-PAGE analysis of recombinant human Galectin-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
Galectin-1 Active Protein | LGALS1 active protein
Human Galectin-1
Gene Names
LGALS1; GBP; GAL1
Reactivity
Human
Purity
>98% by SDS-PAGE & Coomassie stain
Synonyms
Galectin-1; N/A; Human Galectin-1; Recombinant Human Galectin-1; LGALS1 active protein
Host
E Coli
Reactivity
Human
Purity/Purification
>98% by SDS-PAGE & Coomassie stain
Form/Format
lyophilized
Sequence
ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD
Result by N-terminal sequencing:ACGLVASNLNLKPGE
Result by N-terminal sequencing:ACGLVASNLNLKPGE
Sequence Length
134
Endotoxin Level
< 0.1 ng per ug of Galectin-1
Biological Activity
Determine by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2.5 ug/ml with a peak response obatined at 250 ug/ml.
Reconstitution
Human Galectin-I should be reconstituted in water to a concentration of 0.1 mg/mI. This solution can be diluted in water or other buffer solutions or stored at -20 degree C.
Buffer
PBS
Stabilizer
None
Preparation and Storage
The lyophilized human Galectin-l, though stable at room temperature, is best stored desiccated below O degree C. Reconstituted human Galectin-I should be stored in working aliquots at -20 degree C . Avoid repeated freeze and thaw cycles!
SDS-PAGE
(SDS-PAGE analysis of recombinant human Galectin-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
SDS-PAGE
(SDS-PAGE analysis of recombinant human Galectin-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue.)
Related Product Information for LGALS1 active protein
Lectins, of either plant or animal ongm, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. Galectin-I (Gal-I) is one of 15 evolutionarily conserved 13-galactoside-binding proteins that display biologicallydiverse activities in pathogenesis of inflammation and cancer. Gal-I is variably expressed on immune cells and endothelial cells, though is commonly found and secreted at high levels in cancer cells. There is also compelling evidence in models of cancer and autoimmunity that recombinant Gal-I (rGal-l) can potentiate immunoregulatory function of T cells. Galectin-I is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-Ieukemia cell lines through homodimeric binding of Nacetyllactosamines on membrane glycoproteins (Gal-l ligands) and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-l is a 14.5 kDa protein containing 134 amino acid residues.
Product Categories/Family for LGALS1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~14 kDa
NCBI Official Full Name
galectin-1
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 1
NCBI Official Symbol
LGALS1
NCBI Official Synonym Symbols
GBP; GAL1
NCBI Protein Information
galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa
UniProt Protein Name
Galectin-1
UniProt Gene Name
LGALS1
UniProt Synonym Gene Names
Gal-1; HLBP14
UniProt Entry Name
LEG1_HUMAN
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Product Notes
The LGALS1 lgals1 (Catalog #AAA79150) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Human Galectin-1 reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD Result by N-terminal sequencing :ACGLVASNL NLKPGE. It is sometimes possible for the material contained within the vial of "Galectin-1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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