TRAIL/Apo2L, soluble Active Protein | TRAIL/Apo2L active protein
Human TRAIL/Apo2L, soluble
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
Reactivity
Human
Purity
> 98% by SDS-PAGE ans HPLC analysis
Synonyms
TRAIL/Apo2L, soluble; N/A; Human TRAIL/Apo2L, soluble; Recombinant Human TRAIL/Apo2L; TRAIL/Apo2L active protein
Host
E Coli
Reactivity
Human
Purity/Purification
> 98% by SDS-PAGE ans HPLC analysis
Form/Format
Lyophilized
Sequence
MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRS GHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND K QMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFEL KEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG
Sequence Length
168
Endotoxin Level
< 0.1 ng /ug of protein (<1EU/ug).
Biological Activity
Determined by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 0.8 - 2.0 ng/ml.
mRNA RefSeq
NM_003810.3
Preparation and Storage
Stability:
The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 12 months at -20 degree C to -80 degree C.
Reconstitution:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/m. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 12 months at -20 degree C to -80 degree C.
Reconstitution:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/m. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Related Product Information for TRAIL/Apo2L active protein
TRAIL/Apo2L is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptor types belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which activates the cell\'s apoptotic response. Recombinant human soluble TRAIL/Apo2L is a 168 amino acid polypeptide (19.6 kDa), consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL/Apo2L protein.
Product Categories/Family for TRAIL/Apo2L active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19.6 kDa
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 10 isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 10
NCBI Official Symbol
TNFSF10
NCBI Official Synonym Symbols
TL2; APO2L; CD253; TRAIL; Apo-2L
NCBI Protein Information
tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 10
UniProt Gene Name
TNFSF10
UniProt Synonym Gene Names
APO2L; TRAIL; Apo-2L
UniProt Entry Name
TNF10_HUMAN
Similar Products
Product Notes
The TRAIL/Apo2L tnfsf10 (Catalog #AAA79152) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Human TRAIL/Apo2L, soluble reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRS GHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND K QMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFEL KEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG. It is sometimes possible for the material contained within the vial of "TRAIL/Apo2L, soluble, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.