Macrophage Inflammatory protein-1 beta Active Protein | MIP 1b active protein
Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4)
Gene Names
CCL4; ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
Purity
Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Macrophage Inflammatory protein-1 beta; N/A; Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4); MIP 1b Human; Macrophage Inflammatory Protein-1 Beta Human Recombinant (CCL4); Small inducible cytokine A4; CCL4; Macrophage inflammatory protein 1-beta; MIP-1- beta; MIP-1-beta(1-69); T-cell activation protein 2; ACT-2; PAT 744; H400; SIS-gamma; Lymphocyte activation gene 1 protein; LAG-1; HC21; G-26 T-lymphocyte-secreted protein; chemokine (C-C motif) ligand 4; ACT2; G-26; LAG1; MIP1B; SCYA4; AT744.1; MGC104418; MGC126025; MGC126026; MIP 1b active protein
Host
E Coli
Purity/Purification
Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Met-Gly-Ser
Sequence Length
92
Solubility
It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
The Activity is calculated by the ability to chemoattract Human blood monocytes using a concentration of 5-20ng/ml corresponding to a Specific Activity of 50,000-200,000IU/mg.
Preparation and Storage
Lyophilized MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for MIP 1b active protein
Description: Macrophage Inflammatory Protein-1 beta Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7620 Dalton. The CCL4 is purified by proprietary chromatographic techniques.
Introduction: Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b induce synthesis and release of other pro-inflammatory cytokines such as interleukin-1 (IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17.
Introduction: Macrophage Inflammatory Proteins belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1a and MIP-1b that are now also named CCL3 and CCL4. Both factors are produced by macrophages after they are stimulated with bacterial endotoxins. MIP-1a and MIP-1b activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. MIP-1a and MIP-1b induce synthesis and release of other pro-inflammatory cytokines such as interleukin-1 (IL-1), IL-6 and TNF-alpha from fibroblasts and macrophages. CCL3 and CCL4 genes are both located on human chromosome 17.
Product Categories/Family for MIP 1b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,212 Da
NCBI Official Full Name
C-C motif chemokine 4
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 4
NCBI Official Symbol
CCL4
NCBI Official Synonym Symbols
ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
NCBI Protein Information
C-C motif chemokine 4; G-26 T-lymphocyte-secreted protein; MIP-1-beta(1-69); PAT 744; SIS-gamma; T-cell activation protein 2; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; secreted protein G-26; small inducible cytokine A4 (homologous to mouse Mip-1b)
UniProt Protein Name
C-C motif chemokine 4
UniProt Gene Name
CCL4
UniProt Synonym Gene Names
LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
UniProt Entry Name
CCL4_HUMAN
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Product Notes
The MIP 1b ccl4 (Catalog #AAA38083) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Pro-Me t-Gly-Ser. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory protein-1 beta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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