GDNF active protein
Mouse GDNF
Gene Names
Gdnf; AI385739
Reactivity
Mouse
Purity
> 98% by SDS-PAGE and HPLC analyses
Synonyms
GDNF; N/A; Mouse GDNF; Recombinant Murine GDNF; GDNF active protein
Host
E Coli
Reactivity
Mouse
Purity/Purification
> 98% by SDS-PAGE and HPLC analyses
Form/Format
Lyophilized (10mM Sodium Cirate)
Sequence
MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTA IHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS R RLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Sequence Length
240
Endotoxin Level
< 0.1 ng/ug or protein (<1EU/ug)
Structural Information
Disulphide-linked homodimer
Biological Activity
The ED50 was determined by the proliferation of rat C6 cells is <= 0.2 ng/ml, corresponding to a specific activity of >= 5 x 106 units/mg.
Length (aa)
135
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C. For extended storage, itis recommended to further dilute in buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Preparation and Storage
The lyophilized protein is stable at 4°C. Reconstituted working aliquots are stable at 2°C to 8°C and -20°C to -80°C.
Related Product Information for GDNF active protein
GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRalpha (alpha1-alpha4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson\'s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Product Categories/Family for GDNF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15.1 kDa (monomere)
NCBI Official Full Name
glial cell line-derived neurotrophic factor
NCBI Official Synonym Full Names
glial cell line derived neurotrophic factor
NCBI Official Symbol
Gdnf
NCBI Official Synonym Symbols
AI385739
NCBI Protein Information
glial cell line-derived neurotrophic factor; ATF; mGDNF; astrocyte-derived trophic factor
UniProt Protein Name
Glial cell line-derived neurotrophic factor
UniProt Gene Name
Gdnf
UniProt Synonym Gene Names
mGDNF; ATF
UniProt Entry Name
GDNF_MOUSE
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Product Notes
The GDNF gdnf (Catalog #AAA79245) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Mouse GDNF reacts with Mouse and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTA IHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS R RLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI. It is sometimes possible for the material contained within the vial of "GDNF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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