Bone Morphogenetic Protein 7 (BMP7) Active Protein | BMP7 active protein

Active Bone Morphogenetic Protein 7 (BMP7)

Cat. Num. AAA21057

• Gene Names: Bmp7; OP1
• Reactivity: Mus musculus (Mouse)
• Applications: Cell culture, Activity Assays
• Purity: >98%
• Synonyms: Bone Morphogenetic Protein 7 (BMP7); N/A; Active Bone Morphogenetic Protein 7 (BMP7); OP1; Osteogenic Protein-1; Eptotermin alfa; BMP7 active protein

• Host: E. coli
• Reactivity: Mus musculus (Mouse)
• Purity/Purification: >98%
• Form/Format: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 300ug/mL (varies by lot)

• Applicable Applications for BMP7 active protein: Cell culture, Activity Assays
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Ser292~His430
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Predicted isoelectric point: 8.5
• Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Sample: Recombinant BMP7, Mouse;Antibody: Rabbit Anti-Mouse BMP7 Ab)

SDS-PAGE

(Sample: Active recombinant BMP7, Mouse)

Gene Sequencing (extract)

Application Data

(The dose-effect curve of BMP7 was shown in Figure 2. It was obvious that BMP7 significantly promoted cell proliferation of MDA-MB-231 cells. The ED50 for this effect is typically 3.483 to 9.017 ng/mL.)

Activity

(BMP7 (Bone morphogenetic protein 7), which belongs to the TGF-beta superfamily, is a signaling molecule with the ability to promote bone formation. BMP7 has also been implicated in various types of cancer, including breast cancer. It has been reported that BMP7 treatment induced cell growth promotion of MDA-MB-231 breast cancer line. To test the effect of BMP7 on cell proliferation, MDA-MB-231 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of BMP7. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.)

Application Data

Product Categories/Family for BMP7 active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 31982487
• NCBI GeneID: 12162
• NCBI Accession #: NP_031583.2
• NCBI GenBank Nucleotide #: NM_007557.3
• UniProt Accession #: P23359; Q91XF7
• Molecular Weight: Predicted Molecular Mass: 16.9kDa; Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: bone morphogenetic protein 7 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: Bmp7
• NCBI Official Synonym Symbols: OP1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• UniProt Gene Name: Bmp7
• UniProt Synonym Gene Names: Bmp-7; Op1; BMP-7; OP-1