Lecithin Cholesterol Acyltransferase (LCAT) Active Protein | LCAT active protein

Active Lecithin Cholesterol Acyltransferase (LCAT)

Cat. Num. AAA21104

• Gene Names: Lcat; AI046659; D8Wsu61e
• Reactivity: Mus musculus (Mouse)
• Applications: Cell culture; Activity Assays; In vivo assays.
• Purity: >98%
• Synonyms: Lecithin Cholesterol Acyltransferase (LCAT); Active Lecithin Cholesterol Acyltransferase (LCAT); phosphatidylcholine-sterol O-acyltransferase; Phospholipid-cholesterol acyltransferase; LCAT active protein

• Host: E. coli
• Reactivity: Mus musculus (Mouse)
• Purity/Purification: >98%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% sarcosyl and 5% trehalose.

• Applicable Applications for LCAT active protein: Cell culture; Activity Assays; In vivo assays.
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Glu41~His210
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Predicted isoelectric point: 6.3
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Western Blot Sample: Recombinant LCAT, Mouse;Antibody: Rabbit Anti-Mouse LCAT Ab)

SDS-PAGE

(Sample: Active recombinant LCAT, Mouse)

Gene Sequencing (extract)

Activity

(LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was shown in Figure 1 and this effect was in a dose dependent manner.)

Sequence Information

Product Categories/Family for LCAT active protein

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 244791354
• NCBI GeneID: 16816
• NCBI Accession #: NP_032516.2
• NCBI GenBank Nucleotide #: NM_008490.2
• UniProt Accession #: P16301; Q8K139
• Molecular Weight: Predicted Molecular Mass: 20.7kDa; Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: phosphatidylcholine-sterol acyltransferase
• NCBI Official Synonym Full Names: lecithin cholesterol acyltransferase
• NCBI Official Symbol: Lcat
• NCBI Official Synonym Symbols: AI046659; D8Wsu61e
• NCBI Protein Information: phosphatidylcholine-sterol acyltransferase
• UniProt Protein Name: Phosphatidylcholine-sterol acyltransferase
• UniProt Gene Name: Lcat