SDS-PAGE
Benzonase Nuclease Enzyme | nucA enzyme
Benzonase Nuclease
Purity
>95% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining
Synonyms
Benzonase Nuclease; N/A; nucA enzyme
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining
Form/Format
Liquid. 25mM Tris pH 8.0, 100mM NaCI, 50% Glycerol
Tag
C-terminal His
Enzyme Activity
>800U/ul
Unit Definition
37 degree C, pH8.0 reaction conditions, 2.625 mL reaction system, change the absorption value of deltaA260 to 1.0 in 30 minutes (equivalent to the complete digestion of 37ug salmon sperm DNA into an oligonucleotide). The amount of enzyme used is defined as an activity. Unit (U)
Standard Operating Procedure
1. Method
a. Tissue sample processing method: After grinding 30-100mg of animal and plant tissues, add 100-200ul of lysate, and add 5-10ul of lysate at the same time.
Benzonase Nuclease, mix well, incubate at room temperature or on ice for 30min, collect the lysate, centrifuge and take the supernatant for subsequent operations.
b. Cell sample processing method: Collect suspended cells by centrifugation, resuspend the cells by adding 1ml RIPA lysate, and add 10-20ul of
Benzonase Nuclease, mix well, incubate at room temperature or on ice for 30min, collect the lysate, centrifuge and take the supernatant for subsequent operations. Tip: Reattach adherent cells to PBS for post-treatment in the same way as suspended cells.
c. Escherichia coli or other bacteria treatment methods: Collect the bacteria by centrifugation and lysate, and add 1-5ul of Benzonase per 1ml. Nuclease, mix well, incubate at room temperature for 30min, collect lysate, centrifuge and take the supernatant for subsequent operations.
a. Tissue sample processing method: After grinding 30-100mg of animal and plant tissues, add 100-200ul of lysate, and add 5-10ul of lysate at the same time.
Benzonase Nuclease, mix well, incubate at room temperature or on ice for 30min, collect the lysate, centrifuge and take the supernatant for subsequent operations.
b. Cell sample processing method: Collect suspended cells by centrifugation, resuspend the cells by adding 1ml RIPA lysate, and add 10-20ul of
Benzonase Nuclease, mix well, incubate at room temperature or on ice for 30min, collect the lysate, centrifuge and take the supernatant for subsequent operations. Tip: Reattach adherent cells to PBS for post-treatment in the same way as suspended cells.
c. Escherichia coli or other bacteria treatment methods: Collect the bacteria by centrifugation and lysate, and add 1-5ul of Benzonase per 1ml. Nuclease, mix well, incubate at room temperature for 30min, collect lysate, centrifuge and take the supernatant for subsequent operations.
Preparation and Storage
Store at -20°C.
SDS-PAGE
SDS-PAGE
Product Categories/Family for nucA enzyme
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
MW: 29.9kDa
Similar Products
Product Notes
The nucA (Catalog #AAA11074) is an Enzyme produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Benzonase Nuclease, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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