c-AMP dependant Protein Kinase A catalytic subunit alpha Active Protein | PRKACA active protein

Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha

Cat. Num. AAA38382

• Gene Names: PRKACA; PKACA; PPNAD4
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: c-AMP dependant Protein Kinase A catalytic subunit alpha; N/A; Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha; PRKACA; cAMP-Dependent Protein Kinase A catalytic subunit alpha Recombinant; cAMP-dependent protein kinase alpha-catalytic subunit; EC 2.7.11.11; PKA C-alpha; PKACA; MGC48865; MGC102831; PRKACA active protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: PKA catalytic subunit a is supplied in a buffer containing 25mM potassium phosphate (pH 6.5), 5mM 2-mercaptoethanol, 5mM EDTA, 150mM NaCl and 50% glycerol.
• Sequence: MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WESPAQNTAH LDQFERIKTL GTGSFGRVML VKHKETGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE F
• Sequence Length: 351

• Unit Defenition: One unit is defined as the amount of recombinant PKA catalytic subunit a, required to incorporate 1pmol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30 degree C.
• Specific Activity: The specific activity of the recombinant PKA catalytic subunit a, is >15.000.000 U/mg.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Related Product Information for PRKACA active protein

Description: cAMP-dependent PKA is an ubiquitous serine/threonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle, heart). The intracellular cAMP level regulates cellular responses by altering the interaction between the catalytic C and regulatory R subunits of PKA. The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits. Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. Different isoforms of catalytic and regulatory subunits suggest specific functions. The recombinant PKA catalytic subunit a is a 41kDa protein. The a-isoform is the predominant form with a broad tissue distribution and can be used for in vitro enzymological studies of neural and hormonal signal transduction or to phosphorylate target proteins in vivo including Ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism.

Product Categories/Family for PRKACA active protein

Protein Kinases; Enzymes; Protein Kinase-A

NCBI and Uniprot Product Information

• NCBI GI #: 4506055
• NCBI GeneID: 5566
• NCBI Accession #: NP_002721.1
• NCBI GenBank Nucleotide #: NM_002730.3
• UniProt Accession #: P17612; Q32P54; Q9H2Y0; Q9NRB4; Q9NRH9
• Molecular Weight: 39,822 Da
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, catalytic, alpha
• NCBI Official Symbol: PRKACA
• NCBI Official Synonym Symbols: PKACA; PPNAD4
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; protein kinase A catalytic subunit
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• UniProt Gene Name: PRKACA
• UniProt Synonym Gene Names: PKACA; PKA C-alpha
• UniProt Entry Name: KAPCA_HUMAN