BMP-7 protein
BMP-7, Human
Applications
Functional Assay
Purity
>95% as determined by SDS-PAGE. Ni- NTA chromatography.
Synonyms
BMP-7; N/A; BMP-7, Human; BMP-7 protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE. Ni- NTA chromatography.
Applicable Applications for BMP-7 protein
FA (Functional Assay)
Bioactivity
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED 50 for this effect is < 0.65 ug/mL.
Endotoxin
<0.1 EU per 1 ug of the protein by the LAL method
Protein Sequence
MANVAENSSSDQRQACKKHELYVSF RDLGWQDWIIAPEGYAAYYCEGECAF PLNSYMNATNHAIVQTLVHFINPETV PKPCCAPTQLNAISVLYFDDSSNVILK KYRNMVVRACGCH with polyhistidine tag at the C-terminus
Ingredients
Protein lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, and pH 3.5
Usage
1. It is recommended to use sterile water resuspended lyophilized powder, with a solution concentration of no less than 100 ug/mL, not higher than 1mg/mL and let stand at room temperature for at least 20 minutes to fully dissolve. Do not vortex violently.
2. The resuspended solution should be kept sterile at 2-8°C for no more than 1 week.
3. If long-term storage is required, it is recommended to use a sterile solution containing carrier protein (such as 0.1% BSA, 10% FBS, or 5% HSA) for further dilution (not less than 10ug/mL) before aliquot and storage. Avoid repeated freeze/thaw cycles.
2. The resuspended solution should be kept sterile at 2-8°C for no more than 1 week.
3. If long-term storage is required, it is recommended to use a sterile solution containing carrier protein (such as 0.1% BSA, 10% FBS, or 5% HSA) for further dilution (not less than 10ug/mL) before aliquot and storage. Avoid repeated freeze/thaw cycles.
Preparation and Storage
Store at -20°C for 1 year, avoiding freeze/thaw cycles
Related Product Information for BMP-7 protein
BMP-7 (Bone morphogenetic protein 7) is a bone morphogenetic protein which belongs to the TGF - β superfamily. OP-1 is expressed in the brain, kidneys, and bladder. BMP-7 may be involved in bone homeostasis and plays a key role in the transformation of mesenchymal cells into bone and cartilage. The phosphorylation of SMAD1 and SMAD5 can be induced by BMP-7, which in turn induce transcription of numerous osteogenic genes. BMP-7 treatment can also induce all of the genetic markers of osteoblast differentiation in many cell types. Human recombinant BMP-7 protein can be used to aid in the fusion of vertebral bodies to prevent neurologic trauma. It also functions in the treatment of tibial non-union, frequently in cases where a bone graft has failed. It is found that BMP7 has the potential for treatment of chronic kidney disease.
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Product Notes
The BMP-7 (Catalog #AAA268945) is a Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BMP-7 can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay). Researchers should empirically determine the suitability of the BMP-7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP-7, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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