Application Data
Growth Regulated Oncogene Beta (GROb) Active Protein | GROb active protein
Active Growth Regulated Oncogene Beta (GROb)
Gene Names
Cxcl2; Mip-2; Scyb2
Reactivity
Rat
Applications
Activity Assay, Cell Culture
Purity
> 97%
Synonyms
Growth Regulated Oncogene Beta (GROb); N/A; Active Growth Regulated Oncogene Beta (GROb); CXCL2; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Chemokine(C-X-C-Motif)ligand 2; GROb active protein
Host
E Coli
Reactivity
Rat
Purity/Purification
> 97%
Form/Format
Freeze-dried powder
Sequence Length
100
Applicable Applications for GROb active protein
Activity Assay, Cell Culture
Tag
N-terminal His and GST Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Ser32~Asn100
Isoelectric Point
6.7
Application Data
Application Data
SDS-PAGE
SDS-PAGE
Activity
(Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity
(Growth Regulated Oncogene Beta (GROb) is a small cytokine belonging to the CXC chemokine family which produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. Besides, Dipeptidyl Peptidase IV (DPP4) has been identified as an interactor of GROb, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GROb and recombinant rat DPP4. Briefly, GROb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to DPP4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GROb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GROb and DPP4 was shown in Figure 1, and this effect was in a dose dependent manner.)
Product Categories/Family for GROb active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Weight: 37.6kDa
Accurate Molecular Weight: 41kDa
Accurate Molecular Weight: 41kDa
NCBI Official Full Name
C-X-C motif chemokine 2
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 2
NCBI Official Symbol
Cxcl2
NCBI Official Synonym Symbols
Mip-2; Scyb2
NCBI Protein Information
C-X-C motif chemokine 2
UniProt Protein Name
C-X-C motif chemokine 2
UniProt Gene Name
Cxcl2
UniProt Synonym Gene Names
Cinc3; Mip-2; Mip2; Scyb2; CINC-3; MIP2
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Product Notes
The GROb cxcl2 (Catalog #AAA148193) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Active Growth Regulated Oncogene Beta (GROb) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Growth Regulated Oncogene Beta (GROb) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the GROb cxcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Growth Regulated Oncogene Beta (GROb), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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