RANKL, soluble Active Protein | RANKL active protein

Rat RANKL, soluble

Cat. Num. AAA79211

• Gene Names: Tnfsf11; RANKL
• Reactivity: Rat
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Synonyms: RANKL, soluble; N/A; Rat RANKL, soluble; Soluble Receptor Activator of NFkB Ligand; TNFSF11; TRANCE (TNF-related activation-induced cytokine); OPGL; ODF (Osteoclast differentiation factor); RANKL active protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: > 98% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: PAMMEGSWLD VARRGKPEAQ PFAHLTINAA DIPSGSHKVS LSSWYH DRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPA D YLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGF FKL RAGEEISVQV SNPSLLDPDQ DATYFGAFKV QDID
• Sequence Length: 174

• Endotoxin level: <0.1 ng /ug of protein (<1EU/ug)
• Reconsitution: Centrifuge the vial prior to opening.Reconstitute in water to a concentration of 0.1-1.0 mg/m. do not vertex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
• Biological Activity: Determined by its ability to induce NFkappaB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml.
• Preparation and Storage: Stability: The lyophilized protein is stable at room temperature for 1 month and at 4 degree C for 6 months. Reconstituted working aliquots are stable for 1 week at 2 degree C to 8 degree C and for 3 months at -20 degree C to -80 degree C.; Avoid freeze/thaw cycles.

Related Product Information for RANKL active protein

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK + T cells, and to regulate T-cell-dependent immune response. RANKL, which is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Recombinant rat sRANKL is a 19.4 kDa polypeptide comprising the TNF homologous region of RANKL (174 amino acid residues).

Product Categories/Family for RANKL active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 16924012
• NCBI GeneID: 117516
• NCBI Accession #: NP_476490.1
• NCBI GenBank Nucleotide #: NM_057149.1
• UniProt Accession #: Q9ESE2; Q91ZI9
• Molecular Weight: 19.4kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: RANKL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11; ODF; OPGL; TRANCE; osteoprotegerin ligand; osteoclast differentiation factor; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_RAT