Endonuclease G, Mitochondrial (ENDOG) Active Protein | ENDOG active protein

Active Endonuclease G, Mitochondrial (ENDOG)

Cat. Num. AAA148142

• Reactivity: Rattus norvegicus (Rat)
• Applications: Activity Assay
• Purity: >90%
• Synonyms: Endonuclease G, Mitochondrial (ENDOG); N/A; Active Endonuclease G, Mitochondrial (ENDOG); ENDO-G; ENDOG active protein

• Host: E. coli
• Reactivity: Rattus norvegicus (Rat)
• Purity/Purification: >90%
• Form/Format: PBS, pH7.4, containing 0.01% Sarcosyl, 5% Trehalose.
• Concentration: Original Concentration: 350ug/mL (varies by lot)

• Applicable Applications for ENDOG active protein: Activity Assay
• Source: Prokaryotic expression
• Residues: Met1~Lys294
• Tags: N-terminal His and GST Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Predicted isoelectric point: 9.8
• Usage: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS_PAGE

(Sample: Active recombinant ENDOG, Rat)

Gene Sequencing

(Gene Sequencing (extract))

Bioactivity

(Endonuclease G, Mitochondrial (ENDOG) is a conserved nuclease encoded by nuclear DNA and localized in the mitochondrial intermembrane space. It plays dual roles in apoptosis and mitochondrial genome maintenance. During programmed cell death, ENDOG is released into the cytosol, cleaving nuclear and mitochondrial DNA at specific sites to facilitate apoptotic degradation. Structurally, it functions as a homodimer with Mg2+-dependent endonuclease activity. Beyond apoptosis, ENDOG participates in mitochondrial DNA replication, repair, and maternal genome processing during embryogenesis. Notably, ENDOG interacts with cytochrome c (CYCS) to synergistically amplify apoptosis. This complex enhances nucleolytic DNA degradation and modulates the mitochondrial permeability transition pore (MPTP), further promoting cell death.Thus a functional ELISA assay was conducted to detect the interaction of recombinant rat ENDOG and recombinant human CYCS. Briefly, ENDOG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CYCS-coated microtiter wells and incubated for 1h at 37 Degree C. Wells were washed with PBST and incubated for 1h with anti-ENDOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 Degree C, wells were aspirated and washed 5 times.With the addition of substrate solution, wells were incubated 15-25 minutes at 37 Degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat ENDOG and recombinant human CYCS was shown in Figure 1, the EC50 for this effect is 1.01ug/mL.)

Sequence

Product Categories/Family for ENDOG active protein

Enzyme & Kinase; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 77917570
• NCBI GeneID: 362100
• NCBI Accession #: NP_001030110.1
• NCBI GenBank Nucleotide #: NM_001034938.1
• UniProt Accession #: Q3V5X8
• Molecular Weight: Predicted Molecular Mass: 62.3kDa; Accurate Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: endonuclease G, mitochondrial
• NCBI Official Synonym Full Names: endonuclease G
• NCBI Official Symbol: Endog
• NCBI Protein Information: endonuclease G, mitochondrial
• UniProt Protein Name: Endonuclease G
• UniProt Gene Name: Endog