Monocyte Chemotactic Protein-1 Active Protein | rMCP 1 active protein
Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2)
Gene Names
Ccl2; MCP-1; Scya2; Sigje
Purity
Greater than 98.0% as determined by SDS-PAGE.
Synonyms
Monocyte Chemotactic Protein-1; N/A; Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2); MCP 1 Rat; Monocyte Chemotactic Protein-1 Rat Recombinant (CCL2); Small inducible cytokine A2; CCL2; Monocyte chemotactic protein 1; MCP-1; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte secretory protein JE; HC11; chemokine (C-C motif) ligand 2; MCP1; SCYA2; GDCF-2; SMC-CF; HSMCR30; MGC9434; GDCF-2 HC11; Immediate-early serum-responsive JE protein; rMCP 1 active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by SDS-PAGE.
Form/Format
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN
Sequence Length
148
Solubility
It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
ED50 =1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay.
Preparation and Storage
Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for rMCP 1 active protein
Description: Monocyte Chemotactic Protein-1 Rat Recombinant produced in E Coli is a non-glycosylated, Polypeptide chain containing 125 amino acids and having a molecular mass of 14.1 kDa. The MCP-1 is purified by proprietary chromatographic techniques.
Introduction: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B (NF?B). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weightof approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.
Introduction: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B (NF?B). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weightof approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.
Product Categories/Family for rMCP 1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,460 Da
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
Ccl2
NCBI Official Synonym Symbols
MCP-1; Scya2; Sigje
NCBI Protein Information
C-C motif chemokine 2; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic protein 1; small inducible cytokine A2; small inducible gene JE; small-inducible cytokine A2
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
Ccl2
UniProt Synonym Gene Names
Je; Mcp1; Scya2; MCP-1
UniProt Entry Name
CCL2_RAT
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Product Notes
The rMCP 1 ccl2 (Catalog #AAA38086) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN. It is sometimes possible for the material contained within the vial of "Monocyte Chemotactic Protein-1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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