<
WB (Western Blot)
(Western BlotSample: Recombinant TGFb1, Mouse;Antibody: Rabbit Anti-Mouse TGFb1 Ab)
Transforming Growth Factor Beta 1 Active Protein | TGFb1 active protein
Active Transforming Growth Factor Beta 1 (TGFb1)
Gene Names
Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
Reactivity
Mus musculus (Mouse)
Applications
Cell culture; Activity Assays.
Purity
>98%
Synonyms
Transforming Growth Factor Beta 1; N/A; Active Transforming Growth Factor Beta 1 (TGFb1); TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide; TGFb1 active protein
Host
E. coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
>98%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 150ug/mL (varies by lot)
Applicable Applications for TGFb1 active protein
Cell culture; Activity Assays.
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression.
Residues
Ala279~Ser390
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
8.4
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western BlotSample: Recombinant TGFb1, Mouse;Antibody: Rabbit Anti-Mouse TGFb1 Ab)
WB (Western Blot)
(Western BlotSample: Recombinant TGFb1, Mouse;Antibody: Rabbit Anti-Mouse TGFb1 Ab)
SDS-PAGE
(Sample: Active recombinant TGFb1, Mouse)
SDS-PAGE
(Sample: Active recombinant TGFb1, Mouse)
Gene Sequencing
(Figure. Gene Sequencing (Extract))
Gene Sequencing
(Figure. Gene Sequencing (Extract))
Application Data
(Inhibition of A549 cells proliferation after stimulated with TGF-β1.)
Application Data
(Inhibition of A549 cells proliferation after stimulated with TGF-β1.)
Activity
(Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. To test the effect of TGF-β1 on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 5,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant mouseTGF-β1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 2 hours at 37°C. Proliferation of A549 cells after incubation with TGF-β1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse TGF-β1 for 48h. The result was shown in Figure 2. It was obvious that TGF-β1 significantly inhibit cell viability of A549 cells. The ED50 is 7.1ug/mL.)
Activity
(Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. To test the effect of TGF-β1 on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 5,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant mouseTGF-β1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 2 hours at 37°C. Proliferation of A549 cells after incubation with TGF-β1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse TGF-β1 for 48h. The result was shown in Figure 2. It was obvious that TGF-β1 significantly inhibit cell viability of A549 cells. The ED50 is 7.1ug/mL.)
Sequence Information
Sequence Information
Product Categories/Family for TGFb1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 14.1kDa
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
Transforming growth factor beta-1 proprotein
NCBI Official Synonym Full Names
transforming growth factor, beta 1
NCBI Official Symbol
Tgfb1
NCBI Official Synonym Symbols
Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
NCBI Protein Information
transforming growth factor beta-1 proprotein
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
Tgfb1
UniProt Synonym Gene Names
TGF-beta-1; LAP
UniProt Entry Name
TGFB1_MOUSE
Similar Products
Product Notes
The TGFb1 tgfb1 (Catalog #AAA21163) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Transforming Growth Factor Beta 1 (TGFb1) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Transforming Growth Factor Beta 1 can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the TGFb1 tgfb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor Beta 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.