SDS-PAGE
papillomavirus type 18 Protein E6 Recombinant Protein | E6 recombinant protein
Recombinant Human papillomavirus type 18 Protein E6
Applications
Calibrator or standard
Purity
SDS-PAGE >90%
Synonyms
papillomavirus type 18 Protein E6; N/A; Recombinant Human papillomavirus type 18 Protein E6; E6 recombinant protein
Host
E.coli
Purity/Purification
SDS-PAGE >90%
Form/Format
10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Positions
1-158
Sequence
MARFEDPTRR PYKLPDLCTE LNTSLQDIEI TCVYCKTVLE LTEVFEFAFK DLFVVYRDSI PHAACHKCID FYSRIRELRH YSDSVYGDTL EKLTNTGLYN LLIRCLRCQK PLNPAEKLRH LNEKRRFHNI AGHYRGQCHS CCNRARQERL QRRRETQV
Applicable Applications for E6 recombinant protein
Calibrator or standard
Tag Information
his-tag
Appearance
Clear Solution
Preparation and Storage
Store at -20°.
SDS-PAGE
SDS-PAGE
Related Product Information for E6 recombinant protein
Transcriptional transactivator. Binds double-stranded DNA. Has transforming activity. Inactivates, with E6-AP ubiquitin-protein ligase, the human p53/TP53 tumor suppressor protein by targeting it to degradation. Binds and targets human MUPP1/MPDZ protein to degradation. Those two functions presumably contribute to transforming activity. Interaction with human FBLN1 protein also ses to be linked to cell transformation.
Product Categories/Family for E6 recombinant protein
References
Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products.Cole S.T., Danos O.J. Mol. Biol. 193:599-608(1987)
The expression of human papillomavirus type 18 E6 protein in bacteria and the production of anti-E6 antibodies.Matlashewski G., Banks L., Wu-Liao J., Spence P., Pim D., Crawford L.J. Gen. Virol. 67:1909-1916(1986)
Nucleotide sequences of cDNAs for human papillomavirus type 18 transcripts in HeLa cells.Inagaki Y., Tsunokawa Y., Takebe N., Nawa H., Nakanishi S., Terada M., Sugimura T.J. Virol. 62:1640-1646(1988)
Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes.Schneider-Gaedicke A., Schwarz E.EMBO J. 5:2285-2292(1986)
Identification of early proteins of the human papilloma viruses type 16 (HPV 16)and type 18 (HPV 18)in cervical carcinoma cells.Seedorf K., Oltersdorf T., Kraemer G., Roewekamp W.EMBO J. 6:139-144(1987)
E6 protein of human papillomavirus type 18 binds zinc.Grossman S.R., Laimins L.A.Oncogene 4:1089-1093(1989)
The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53.Scheffner M., Huibregtse J.M., Vierstra R.D., Howley P.M.Cell 75:495-505(1993)
Multi-PDZ domain protein MUPP1 is a cellular target for both adenovirus E4-ORF1 and high-risk papillomavirus type 18 E6 oncoproteins.Lee S.S., Glaunsinger B., Mantovani F., Banks L., Javier R.T.J. Virol. 74:9680-9693(2000)
Interaction of oncogenic papillomavirus E6 proteins with fibulin-1.Du M., Fan X., Hong E., Chen J.J.Biochem. Biophys. Res. Commun. 296:962-969(2002)
Oncogenic human papillomavirus E6 proteins target the MAGI-2 and MAGI-3 proteins for degradation.Thomas M., Laura R., Hepner K., Guccione E., Sawyers C., Lasky L., Banks L.Oncogene 21:5088-5096(2002)
Role of the PDZ domain-binding motif of the oncoprotein E6 in the pathogenesis of human papillomavirus type 31.Lee C., Laimins L.A.J. Virol. 78:12366-12377(2004)
HPV E6 specifically targets different cellular pools of its PDZ domain-containing tumour suppressor substrates for proteasome-mediated degradation.Massimi P., Gammoh N., Thomas M., Banks L.Oncogene 23:8033-8039(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
E6 protein
NCBI Official Symbol
E6
NCBI Protein Information
E6 protein
UniProt Protein Name
Protein E6
UniProt Gene Name
E6
UniProt Entry Name
VE6_HPV18
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Product Notes
The E6 e6 (Catalog #AAA18688) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-158 with tag his-tag. AAA Biotech's papillomavirus type 18 Protein E6 can be used in a range of immunoassay formats including, but not limited to, Calibrator or standard. Researchers should empirically determine the suitability of the E6 e6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MARFEDPTRR PYKLPDLCTE LNTSLQDIEI TCVYCKTVLE LTEVFEFAFK DLFVVYRDSI PHAACHKCID FYSRIRELRH YSDSVYGDTL EKLTNTGLYN LLIRCLRCQK PLNPAEKLRH LNEKRRFHNI AGHYRGQCHS CCNRARQERL QRRRETQV. It is sometimes possible for the material contained within the vial of "papillomavirus type 18 Protein E6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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