Standard Curve (Sample)
(Fig.1. Human Excitatory amino acid transporter 1 (SLC1A3) Standard Curve.)
Human Excitatory Amino Acid Transporter 1 (SLC1A3) ELISA Kit | EAAT1 elisa kit
Human Excitatory Amino Acid Transporter 1 (SLC1A3) ELISA Kit
Gene Names
SLC1A3; EA6; EAAT1; GLAST; GLAST1
Reactivity
Human
Synonyms
Excitatory Amino Acid Transporter 1 (SLC1A3); N/A; Human Excitatory Amino Acid Transporter 1 (SLC1A3) ELISA Kit; SLC1A3; EA6; FLJ25094; GLAST; GLAST1; excitatory amino acid transporter 1; sodium-dependent glutamate/aspartate transporter; EAAT1 elisa kit
Reactivity
Human
Specificity
Human Excitatory amino acid transporter 1 (SLC1A3) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC1A3. No significant cross-reactivity or interference between Human SLC1A3 and analogues was observed.
Sequence Length
496
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Excitatory amino acid transporter 1 (SLC1A3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Excitatory amino acid transporter 1 (SLC1A3) Standard Curve.)
Related Product Information for EAAT1 elisa kit
Description: This Human Excitatory amino acid transporter 1 (SLC1A3) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC1A3 in samples. An antibody specific for SLC1A3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC1A3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC1A3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC1A3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Glutamate and aspartate are excitatory neurotransmitters that have been implicated in a number of pathologic states of the nervous system. Accumulation of extracellular excitatory amino acids can be cytotoxic and may also lower the seizure threshold in epilepsy. EAAT1 (SLC1A3) is a member of a family of high-affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. The cDNA encoded a deduced protein that was 95% homologous to a previously reported rabbit glutamate/aspartate transporter. The highest expression was found in the substantia nigra, red nucleus, and hippocampus, and in cerebral cortical layers
Background: Glutamate and aspartate are excitatory neurotransmitters that have been implicated in a number of pathologic states of the nervous system. Accumulation of extracellular excitatory amino acids can be cytotoxic and may also lower the seizure threshold in epilepsy. EAAT1 (SLC1A3) is a member of a family of high-affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. The cDNA encoded a deduced protein that was 95% homologous to a previously reported rabbit glutamate/aspartate transporter. The highest expression was found in the substantia nigra, red nucleus, and hippocampus, and in cerebral cortical layers
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
excitatory amino acid transporter 1 isoform 4
NCBI Official Synonym Full Names
solute carrier family 1 member 3
NCBI Official Symbol
SLC1A3
NCBI Official Synonym Symbols
EA6; EAAT1; GLAST; GLAST1
NCBI Protein Information
excitatory amino acid transporter 1
UniProt Protein Name
Excitatory amino acid transporter 1
UniProt Gene Name
SLC1A3
UniProt Synonym Gene Names
EAAT1; GLAST; GLAST1; GLAST-1
UniProt Entry Name
EAA1_HUMAN
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Product Notes
The Human EAAT1 slc1a3 (Catalog #AAA330828) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330828 ELISA Kit recognizes Human EAAT1. It is sometimes possible for the material contained within the vial of "Excitatory Amino Acid Transporter 1 (SLC1A3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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