Standard Curve (Sample)
Human Endothelin-1 ELISA Kit | EDN1 elisa kit
Human Endothelin-1 ELISA Kit
Gene Names
EDN1; ET1; QME; PPET1; ARCND3; HDLCQ7
Reactivity
Human
Synonyms
Endothelin-1; N/A; Human Endothelin-1 ELISA Kit; ET-1/EDN1/ET1/HDLCQ7/PPET1/Preproendothelin-1/endothelin 1/endothelin-1/preproendothelin-1; EDN1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of EDN1. No significant cross-reactivity or interference between EDN1 and analogues was observed.
Sequence Length
211
Assay Type
Competitive
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
1.25-80pg/ml
Sensitivity
0.75pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for EDN1 elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,425 Da
NCBI Official Full Name
endothelin-1 isoform 2 preproprotein
NCBI Official Synonym Full Names
endothelin 1
NCBI Official Symbol
EDN1
NCBI Official Synonym Symbols
ET1; QME; PPET1; ARCND3; HDLCQ7
NCBI Protein Information
endothelin-1
UniProt Protein Name
Endothelin-1
UniProt Gene Name
EDN1
UniProt Synonym Gene Names
PPET1; ET-1
UniProt Entry Name
EDN1_HUMAN
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Product Notes
The Human EDN1 edn1 (Catalog #AAA17441) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17441 ELISA Kit recognizes Human EDN1. It is sometimes possible for the material contained within the vial of "Endothelin-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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