Human ephrin-A1 ELISA Kit | EFNA1 elisa kit

Human Ephrin-A1, EFNA1 ELISA Kit

Cat. Num. AAA113051

• Gene Names: EFNA1; B61; EFL1; ECKLG; EPLG1; LERK1; LERK-1; TNFAIP4
• Reactivity: Human
• Synonyms: ephrin-A1; N/A; Human Ephrin-A1, EFNA1 ELISA Kit; Human Ephrin-A1 (EFNA1) ELISA kit; B61; ECKLG; EFL1; EPLG1; LERK1; TNFAIP4; eph-related receptor tyrosine kinase ligand 1; ephrin A1; immediate early response protein B61; ligand of eph-related kinase 1; tumor necrosis factor; alpha-indu; EFNA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human EFNA1. No significant cross-reactivity or interference between human EFNA1 and analogues was observed.
• Sequence Length: 205
• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of human EFNA1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for EFNA1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EFNA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFNA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 33359682
• NCBI GeneID: 1942
• NCBI Accession #: NP_004419.2
• NCBI GenBank Nucleotide #: NM_004428.2
• UniProt Accession #: P20827; Q5SR60; Q5SR61; Q6I9T9; Q8N578; D3DV86
• Molecular Weight: 23,787 Da
• NCBI Official Full Name: ephrin-A1 isoform a
• NCBI Official Synonym Full Names: ephrin-A1
• NCBI Official Symbol: EFNA1
• NCBI Official Synonym Symbols: B61; EFL1; ECKLG; EPLG1; LERK1; LERK-1; TNFAIP4
• NCBI Protein Information: ephrin-A1; TNF alpha-induced protein 4; ligand of eph-related kinase 1; immediate early response protein B61; eph-related receptor tyrosine kinase ligand 1; tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4
• UniProt Protein Name: Ephrin-A1
• UniProt Gene Name: EFNA1
• UniProt Synonym Gene Names: EPLG1; LERK1; TNFAIP4; LERK-1; TNF alpha-induced protein 4
• UniProt Entry Name: EFNA1_HUMAN