Application Data
(Figure. Cell proliferation of 3T3 cells after stimulated with EGF.)
Epidermal Growth Factor Active Protein | EGF active protein
Active Epidermal Growth Factor (EGF)
Gene Names
Egf; AI790464
Applications
Activity Assay, Cell Culture
Purity
>95%
Synonyms
Epidermal Growth Factor; N/A; Active Epidermal Growth Factor (EGF); URG; Beta-Urogastrone; Pro-epidermal growth factor; EGF active protein
Purity/Purification
>95%
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence Positions
Asn977~Arg1029
Sequence Length
1217
Applicable Applications for EGF active protein
Activity Assay, Cell Culture
Species
Mouse
Tag
N-terminal His and GST Tag
Isoelectric Point
6.3
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
(Figure. Cell proliferation of 3T3 cells after stimulated with EGF.)
Application Data
(Figure. Cell proliferation of 3T3 cells after stimulated with EGF.)
WB (Western Blot)
(Figure. Western Blot)
WB (Western Blot)
(Figure. Western Blot)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for EGF active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 36.0kDa
Accurate Molecular Mass: 38kDa
Accurate Molecular Mass: 38kDa
NCBI Official Full Name
pro-epidermal growth factor isoform 1 preproprotein
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
Egf
NCBI Official Synonym Symbols
AI790464
NCBI Protein Information
pro-epidermal growth factor
UniProt Protein Name
Pro-epidermal growth factor
UniProt Gene Name
Egf
UniProt Synonym Gene Names
EGF
UniProt Entry Name
EGF_MOUSE
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Product Notes
The EGF egf (Catalog #AAA150091) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Asn977~Arg1029. AAA Biotech's Epidermal Growth Factor can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the EGF egf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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