Guinea pig Epidermal growth factor ELISA Kit | EGF elisa kit
Guinea pig Epidermal growth factor (EGF) ELISA Kit
Gene Names
EGF; URG; HOMG4
Reactivity
Guinea pig
Synonyms
Epidermal growth factor; N/A; Guinea pig Epidermal growth factor (EGF) ELISA Kit; URG; Beta-Urogastrone; Pro-epidermal growth factor; EGF elisa kit
Reactivity
Guinea pig
Specificity
This assay has high sensitivity and excellent specificity for detection of Guinea pig EGF. No significant cross-reactivity or interference between Guinea pig EGF and analogues was observed.
Sequence Length
53
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
3.12 pg/mL - 200 pg/mL
Sensitivity
1.56 pg/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for EGF elisa kit
Background: Epidermal growth factor (EGF) was discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. EGF is a member of a family of growth factors that bind to the same 170 kDa receptor, including TGF-, vaccinia growth factor and amphiregulin. EGF is initially synthesized as a large (130 kDa) precursor molecule in which the mature, soluble EGF sequence (6 kDa) is located. The precursor, which functions as a source for soluble EGF, may also have a role in mediation of intercellular communication between cells displaying pro-EGF on their surfaces and cells with EGF receptors.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EGF in Guinea pig serum, plasma. An antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Intended Uses: For the quantitative detection of Guinea pig Epidermal growth factor (EGF) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EGF in Guinea pig serum, plasma. An antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Intended Uses: For the quantitative detection of Guinea pig Epidermal growth factor (EGF) concentration in serum, plasma and other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
129,746 Da
NCBI Official Full Name
epidermal growth factor, partial
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
EGF
NCBI Official Synonym Symbols
URG; HOMG4
NCBI Protein Information
pro-epidermal growth factor
UniProt Protein Name
Pro-epidermal growth factor
UniProt Gene Name
EGF
UniProt Synonym Gene Names
EGF
UniProt Entry Name
EGF_HUMAN
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Product Notes
The Guinea pig EGF egf (Catalog #AAA55871) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA55871 ELISA Kit recognizes Guinea pig EGF. It is sometimes possible for the material contained within the vial of "Epidermal growth factor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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