Epidermal Growth Factor Recombinant Protein | EGF recombinant protein

Human Epidermal Growth Factor Recombinant

Cat. Num. AAA76082

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Epidermal Growth Factor; N/A; Human Epidermal Growth Factor Recombinant; egf; epidermal growth factor; EGF recombinant protein

• Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Epidermal Growth Factor was lyophilized from a 0.2um filtered PBS solution, pH 7.2 with 5% trehalose and 5% mannitol
• Sequence: REDDHHYSVR NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELRHAGHGQQ QKVIVVAVCV VVLVMLLLLS LWGAHYYRTQ KLLSKNPKNP YEESSRDVRS RRPADTEDGM SSCPQPWFVV IKEHQDLKNG GQPVAGEDGQ AADGSMQPTS WRQEPQLCGM GTEQGCWIPV SSDKGSCPQV MERSFHMPSY GTQTLEGGVE KPHSLLSANP LWQQRALDPP HQMELTQ
• Sequence Length: 1165

• Source: Optimized DNA sequence encoding Human EGF mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Biological Activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 0.5 ng/ml, corresponding to a specific activity of >2 x 10^7 units/mg
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Product Categories/Family for EGF recombinant protein

Cytokines

References

• thrombin induces EGF receptor expression and cell proliferation via a pkc () /c-src-dependent pathway in vascular smooth muscle cells .Arterioscler Thromb Vasc Biol, Oct 2009; 29: 1594 - 1601.
• dependence of EGF-induced increases in corneal epithelial proliferation and migration on gsk-3 inactivation .Invest. Ophthalmol. Vis. Sci., Oct 2009; 50: 4828 - 4835.
• internalization and intracellular sorting of the EGF receptor: a model for understanding the mechanisms of receptor trafficking .J. Cell Sci., Oct 2009; 122: 3433 - 3439.
• angiotensin ii maintains cerebral vascular relaxation via EGF receptor transactivation and erk1/2 .Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1296 - H1303.
• g protein-coupled receptor 30 expression is up-regulated by EGF and tgf in estrogen receptor -positive cancer cells .Mol. Endocrinol., Sep 2009; 10.1210/me.2009-0120.